| 头孢克洛血药浓度测定及其药代动力学研究 |
| |
| 引用本文： | 杨生,张宏文,王蔚青,潘雯澜. 头孢克洛血药浓度测定及其药代动力学研究[J]. 中国生化药物杂志, 2008, 29(5) |
| |
| 作者姓名： | 杨生 张宏文 王蔚青 潘雯澜 |
| |
| 作者单位： | 1. 江苏省润天生化医药有限公司,江苏,南京,210036
2. 南京医科大学,第一附属医院,药物研究室,江苏,南京,210029 |
| |
| 摘 要： | 目的建立高效液相色谱法测定人血浆中头孢克洛浓度的方法。方法以头孢拉定为内标,采用DIS-COVERYC18色谱柱(4.6mm×250mm,5μm),以醋酸盐缓冲液-乙腈(83∶17)为流动相,流速:1mL/min;检测波长264nm。结果头孢克洛的线性范围为0.2～30μg/mL,回归方程为Y=0.0913X-0.0094,r=0.9996,最低检测浓度为0.1μg/mL,日内和日间RSD均小于7.5%。结论此法准确简便,适用于头孢克洛药代动力学的研究。
|
| 关 键 词： | 头孢克洛 血药浓度 药代动力学 高效液相色谱法 |
HPLC determination of cefaclor in human plasma and its application in pharmacokinetic studies |
| |
| YANG Sheng,ZHANG Hong-wen,WANG Wei-qing,PAN Wen-lan. HPLC determination of cefaclor in human plasma and its application in pharmacokinetic studies[J]. Chinese Journal of Biochemical Pharmaceutics, 2008, 29(5) |
| |
| Authors: | YANG Sheng ZHANG Hong-wen WANG Wei-qing PAN Wen-lan |
| |
| Affiliation: | YANG Sheng1,ZHANG Hong-wen2,WANG Wei-qing2,PAN Wen-lan2 |
| |
| Abstract: | Purpose A HPLC method was established for the determination of cefaclor in human plasma.Methods Cefaclor and the internal standard cefradine were separated on DISCOVERY C18 column(4.6 mm×250 mm,5 μm)and detected by UV detector.The detection wavelength was 264 nm.The mobile phase consisted of ammonium acetate and acetonitrile(83:17)at a flow rate of 1.0 mL/min.ResultsThe range of calibration was 0.2-30 μg/mL(Y=0.091 3 X-0.009 4,r=0.999 6).The minimal detectable drug concentration in plasma was 0.1 μg/mL.Intra-day RSD and intre-day RSD was <7.5%.ConclusionThe method is simple,rapid and sensitive for cefaclor pharmacokinetics study. |
| |
| Keywords: | cefaclor plasma concentration pharmacokinetics HPLC |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
