E-钙黏附素基因甲基化与结直肠癌临床病理特征的关系

目的探讨E-钙黏附素（E-cadherin）基因甲基化与结直肠癌临床病理特征的关系。方法采用甲基化特异性巢式PCR技术（nMSP法）研究100例结直肠癌组织及100例癌旁组织E-eadherin基因甲基化与临床病理特征的关系。结果100例结直肠癌组织中有33例呈E-cad基因甲基化阳性（阳性率33.0％），相应的癌旁组织无甲基化。Dukes分期中C、D期组织E-cad基因甲基化阳性率（72.7％）明显高于A、B期（27.3％）（P〈0.05）；伴肝转移的结直肠癌组织甲基化阳性率（81．8％）明显高于无肝转移者（18．2％）（P〈0．01）；在肿瘤细胞分化程度、大体类型、年龄、性别和肿瘤部位等其他病理特征，E-cad基因甲基化阳性组与阴性组差异无统计学意义（P〉0．05）。结论结直肠癌组织E-cad基因甲基化与淋巴结转移、肝转移和Dukes分期明显相关，E-cad基因甲基化可能是结直肠癌侵袭性增强的原因之一。

Relationship between E-cadherin gene methylation and clinicopathological characteristics of colorectal cancer

Objective To investigate the relationship between methylation in the promoter of the E-cadherin gene and clinicopathological characteristics of the colorectal cancer.Methods Nest methylation-specific PCR(MSP-UMP) was used to detect E-cadherin methylation of 100 pieces of colorectal cancer specimens,and analyze its relation with clinicopathological characteristics.Results In 100 sporadic colorectal cancer specimens,33% had detectable E-cadherin methylation,but no detectable methylation was found in all normal tissues.The positive rate of E-cadherin methylation in the patients with Dukes C and D was significantly higher than that in those with Dukes A and B((P<)(0.05).)The positive rate of E-cadherin methylation in the patients with liver metastasis was obviously higher than in those without liver metastasis((P<)(0.01).)There was no statistically significant difference in orther pathological characteristics between E-cadherin methylation positive group and negative group((P>)(0.05).)Conclusion Methylation in the promoter of the E-cadherin gene was obviously related with lymph node,liver metastasis and Dukes stage.Methylation of E-cadherin gene may be one of the reasons of the increased invasion of the colorectal cancer.