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| 人水通道蛋白1的B细胞表位初步预测分析 | |
| 引用本文： | 张新颜,顾长国,匡永勤,顾建文,孟岳良.人水通道蛋白1的B细胞表位初步预测分析[J].西南国防医药,2011,21(11):1173-1175,1277. |
| 作者姓名： | 张新颜 顾长国 匡永勤 顾建文 孟岳良 |
| 作者单位： | 1. 成都军区总医院博士后工作站,成都,610083 2. 解放军452医院检验科 3. 成都军区总医院神经外科 4. 成都军区卫生信息中心 |
| 摘 要： | 目的分析人水通道蛋白1的B细胞优势表位，为后续研究获得其单克隆抗体做好铺垫。方法分别对人水通道蛋白1进行亲水性、二级结构、柔韧性、极性、可及性、电荷分布等多参数进行分析，然后用抗原性指数的方法综合分析。结果人水通道蛋白1的肽段21—39、39-45、42～49、79～93、110～125、122～128、130～138、153～164、229—237预测结果具有较高的免疫原性。结论多参数预测分析获得多个人水通道蛋白1表位多肽，为后续制备抗人水通道蛋白1单克隆抗体奠定了基础。 |
| 关 键 词： | 人水通道蛋白1 B细胞 表位预测 免疫原性 |
B cell epitope of human aquaporin protein 1: preliminary analysis and predication |
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| Zhang Xinyan,Gu Changguo,Kuang Yongqin,Gu Jianwen,Meng Yueliang.B cell epitope of human aquaporin protein 1: preliminary analysis and predication[J].Medical Journal of National Defending forces in Southwest China,2011,21(11):1173-1175,1277. | |
| Authors: | Zhang Xinyan Gu Changguo Kuang Yongqin Gu Jianwen Meng Yueliang |
| Institution: | Zhang Xinyan1,Gu Changguo2,Kuang Yongqin3,Gu Jianwen3,Meng Yueliang4 Zhang Xinyan and Gu Changguo are the first authors who contributed equally to the article.1.Postdoctoral station,3.Department of Neurosurgery,General Hospital of Chengdu Military Command,Chengdu,Sichuan,610083,China,2.Department of Laboratory,Hospital 452 of PLA,610021,4.Health Information Center of Chengdu Military Command,610015 |
| Abstract: | Objective To identify the B cell dominant epitope of human aquaporin protein 1(hAQP-1),and to establish basis for obtaining monoclonal antibody to hAQP-1 in the subsequent research.Methods Multiparameters such as hydrophilicity,secondary structure,flexibility,polarity,accessibility,and charge distribution of the hAQP-1 were synthetically analyzed by means of antigenic index method.Results The peptide of 21-39,39-45,42-49,79-93,110-125,122-128,130-138,153-164 and 229-237 in hAQP-1 showed high immunogenicity.... |
| Keywords: | human aquaporin protein 1 B cell epitope prediction immunogenicity |
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