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| 人骨形成蛋白2的基因克隆和序列分析 | |
| 引用本文： | 王德利,阮狄克,张惠中,石峥,范清宇. 人骨形成蛋白2的基因克隆和序列分析[J]. 中国骨与关节损伤杂志, 2004, 19(2): 101-103 |
| 作者姓名： | 王德利 阮狄克 张惠中 石峥 范清宇 |
| 作者单位： | 海军总医院骨外科,北京市,100037;第四军医大学唐都医院骨研所 |
| 基金项目： | 海军后勤部卫生部基金资助 (编号 :0 3HW 1 8) |
| 摘 要： | 目的 探讨克隆人骨形成蛋白 2基因编码区的方法。方法 由人末梢血白细胞中提取细胞总DNA ,利用PCR法 ,从DNA中分别扩增出人骨形成蛋白 2外显子编码区基因DNA片段 ;将获得的基因片段插入PGEM -T -Easy质粒中 ,转化到大肠杆菌DH5α后挑选阳性克隆 ,利用限制性内切酶酶切核苷酸序列分析鉴定后 ,连接获得人骨形成蛋白 2基因。结果 通过质粒DNA酶切分析及序列测定 ,我们获得的基因片段为人骨形成蛋白 - 2DNA序列。结论 首次由人白细胞DNA中克隆获得人骨形成蛋白 2全长基因 ,为表达、应用骨形成蛋白 2提供了前提条件 |
| 关 键 词： | 骨形成蛋白 基因克隆 |
| 修稿时间： | 2003-10-10 |
Cloning of Human Bone Morphogenetic Protein 2 Gene |
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| Wang Deli,Ruan Dike,Zhang Huizhong,et al.. Cloning of Human Bone Morphogenetic Protein 2 Gene[J]. Chinese Journal of Bone and Joint Injury, 2004, 19(2): 101-103 | |
| Authors: | Wang Deli Ruan Dike Zhang Huizhong et al. |
| Affiliation: | Wang Deli,Ruan Dike,Zhang Huizhong,et al.the Orthopeadic Department of Nevy General Hospital 100037 |
| Abstract: | Objective The purpose of this study is to clone human BMP2 DNA.Methods Using Blood cell to extract cell total DNA,human BMP2 DNA was amplified by PCR.The human BMP2 DNA was then inserted into cloning vector pGEM-T-Easy.Screening by white and blue clone after transforming the recombinant plasmid into E.coli,we got the recombinant plasmid which was confirmed by restriction enzyme and sequence analysis.Results Restriction enzyme assay and sequencing showed that the construct we got was human BMP2 DNA.Conclusion We obtained human BMP2 DNA which provide us useful target for further study. |
| Keywords: | Bone mophogenetic protein Gene cloning |
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