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| 嗅鞘细胞移植修复大鼠脊髓损伤的组织病理学变化 | |
| 引用本文： | 王国毓,贺西京,袁普卫,李浩鹏,常瑞. 嗅鞘细胞移植修复大鼠脊髓损伤的组织病理学变化[J]. 中国组织工程研究与临床康复, 2010, 14(6). DOI: 10.3969/j.issn.1673-8225.2010.06.021 |
| 作者姓名： | 王国毓 贺西京 袁普卫 李浩鹏 常瑞 |
| 作者单位： | 西安交通大学第二医院骨二科,陕西省西安市,710004 |
| 摘 要： | 背景:对于脊髓损伤,目前临床尚无有效的治疗对策,近年来嗅鞘细胞移植对脊髓损伤修复取得了一定的进展.目的:观察嗅鞘细胞移植在缓解损伤脊髓的病理反应和超微结构变化,及其在发生发展中的作用.方法:60只大鼠随机分为空白组,模型组,嗅鞘胞移植组和DF12组,每组15只.空白组:仅切开T_(10)全椎板及T_9,T_(11)部分椎板,对脊髓未作其他处理,明胶海绵轻柔压迫止血;模型组:仅切断脊髓,未作特殊处理;嗅鞘细胞移植组和DF12组:切断脊髓后用微量注射器分别注射嗅鞘细胞和DF12培养液,随后缝合切口.脊髓损伤后1,3,7,14,28,42,56 d每组麻醉2只受检大鼠,取材做光镜观察和电镜观察.结果与结论:单纯脊髓横切损伤后,发生了出血、水肿、变性、坏死以及囊腔形成,胶质细胞增生和神经纤维再生.嗅鞘细胞移植后,明显减轻了神经元和神经纤维的坏死变性程度,减轻病理反应,并能对损伤神经元实施保护;防止了胶质细胞过度增生形成瘢痕屏障,明显增加了再生神经纤维的数量.提示嗅鞘细胞移植对损伤脊髓具有减轻病理反应和促进修复的作用. |
| 关 键 词： | 嗅鞘细胞 移植 脊髓损伤 形态学 病理学 大鼠 |
Histopathological changes of rat injured spinal cord following olfactory ensheathing cell transplantation |
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| Wang Guo-yu,He Xi-jing,Yuan Pu-wei,Li Hao-peng,Chang Rui. Histopathological changes of rat injured spinal cord following olfactory ensheathing cell transplantation[J]. Journal of Clinical Rehabilitative Tissue Engineering Research, 2010, 14(6). DOI: 10.3969/j.issn.1673-8225.2010.06.021 | |
| Authors: | Wang Guo-yu He Xi-jing Yuan Pu-wei Li Hao-peng Chang Rui |
| Affiliation: | Wang Guo-yu,He Xi-jing,Yuan Pu-wei,Li Hao-peng,Chang Rui Second Department of Orthopedics,Second Hospital of Xi'an Jiaotong University,Xi'an 710004,Shaanxi Province,China |
| Abstract: | BACKGROUND:There are no effective treatments for spinal cord injury.Transplantation of olfactory ensheathing cells (OECs) has achieved great progress in repairing spinal cord injury.OBJECTIVE:To observe the effect of OECs transplantation on pathological and ultrastructural alterations of spinal cord,and the role in spinal cord injury developing.METHODS:A total of 60 SD rats were randomly divided into blank,model,transplantation and DF12 groups,with 15 animals in each group.The entire vertebral plate of T10,... |
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