PM2.5对BEAS-2B细胞脂质过氧化损伤作用

目的 用大气细颗粒物(PM_2.5)刺激人支气管上皮细胞(BEAS-2B细胞),探讨其脂质过氧化作用。方法 PM_2.5采自河南省郑州市;实验细胞为人支气管上皮细胞。用0、12.5、25 μg/mL的PM_2.5刺激BEAS-2B细胞4 h后,使用流式细胞仪检测其活性氧(ROS)水平;刺激BEAS-2B细胞8 h,用丙二醛试剂盒和超氧化物歧化酶活性测定试剂盒分别检测细胞裂解液中丙二醛(MDA)含量和超氧化物歧化酶(SOD)活性。结果 PM_2.5作用于BEAS-2B细胞4 h,12.5、25 μg/mL染毒组ROS水平分别为(6 074.69±41.65)、(7 338.58±168.34),均明显高于对照组的(5 816.66±114.69)(P<0.01);刺激BEAS-2B细胞8 h,12.5、25 μg/mL染毒组MDA含量分别为(195.44±35.58)、(334.11±26.75) μmol/mg,均明显高于对照组的(71.14±4.21) μmol/mg(P<0.01),同时染毒组SOD活力分别为(100.08±7.54)、(80.03±7.61) U/mg,均低于对照组的(159.91±10.59) U/mg(P<0.01)。结论 PM_2.5可以引起BEAS-2B细胞脂质过氧化损伤。

Lipid peroxidation induced by PM2.5 in human bronchial epithelial cells

Objective To examine the effect of particulate matter less than 2.5 μm in aerodynamic diameter(PM_2.5)on lipid peroxidation in human bronchial epithelial cells(BEAS-2B).Methods PM_2.5 was collected in Zhengzhou city of Henan province; BEAS-2B cells were exposed to the PM_2.5 at the doses of 0,12.5,and 25 μg/ml,respectively.After 4 hours,the level of reactive oxygen species(ROS)was measured with flow cytometer; the amount of malonaldehyde(MDA)and the activity of superoxide dismutase(SOD)in cell lysate were measured with MDA and SOD kit 8 hours after PM_2.5 exposure.Results The levels of ROS in BEAS-2B cells treated with PM_2.5 were 6 074.69±41.65 for the dose of 12.5 μg/ml,7 338.58±168.34 for the dose of 25 μg/ml and were higher than that in the control(5816.66±114.69);the amounts of MDA for the two PM_2.5 exposure groups(195.44±35.58 and 334.11±26.75 μmol/mg) were higher than that in the control group(71.14±4.21 μmol/mg);the activity of SOD in exposure groups(100.08±7.54,80.03±7.61 U/mg) were significantly decreased compared to that in the control group(159.91±10.59 U/mg).Conclusion PM_2.5 exposure could induce lipid peroxidation in BEAS-2B cells.