白藜芦醇对小鼠脂代谢及相关蛋白表达影响

目的探讨白藜芦醇（RSV）对高脂饲料喂养的C57BL/6J小鼠脂代谢及肝脏沉默信息调节因子1（SIRT1）及肝X受体α（LXRα）表达影响。方法雄性C57BL/6J小鼠30只，分为对照组、高脂组和RSV干预组（n=10），对照组和高脂组分别喂饲基础饲料和高脂饲料，RSV干预组在喂饲高脂饲料同时给予RSV灌胃，第16周测定血清甘油三酯（TG）、总胆固醇（TC）、低密度脂蛋白胆固醇（LDL-C）和高密度脂蛋白胆固醇（HDL-C）含量，分离小鼠肝脏，测定TG、TC含量以及SIRT1、LXRα蛋白表达水平。结果与对照组比较，高脂组小鼠血清TG、TC、LDL-C含量[分别为（1.25±0.25）、（4.29±0.59）、（1.59±0.20）mmol/L]和肝脏TG、TC含量[分别为（5.78±1.69）、（2.69±1.20）mmol/L]升高，肝脏SIRT1、LXRα蛋白表达水平[分别为（0.36±0.03）、（0.78±0.07）]下降（P<0.05）；与高脂组比较，RSV干预组小鼠血清TG、TC、LDL-C含量[分别为（0.91±0.15）、（3.65±0.36）、（1.39±0.11）mmol/L]和肝脏TG、TC含量[分别为（4.63±1.70）、（1.65±0.89）mmol/L]明显下降，肝脏SIRT1、LXRα蛋白表达水平[分别为（0.41±0.05）、（0.88±0.09）]明显升高（P<0.05）。结论白藜芦醇可有效改善高脂饲料喂养C57BL/6J小鼠脂代谢异常，其机制可能与促进肝脏SIRT1及LXRα表达有关。

Effect of resveratrol on lipid metabolism and related protein expression in mice

ObjectiveTo investigate the effect of resveratrol (RSV)on lipid metabolism,liver silent information regulator 1 (SIRT1),and liver X receptor α (LXRα)expression in high-fat diet C57BL/6J mice.MethodsThirty male C57BL/6J mice were divided into control group,high-fat group and intervention group (n=10 for each group);the mice in control group and high-fat diet group were fed with standard or high fat diet,and the mice in intervention group were fed with high fat diet and given RSV by gavage at the same time.After 16 weeks,serum triglyceride(TG),total cholesterol (TC),low density lipoprotein-cholesterol (LDL-C)and high density lipoprotein-cholesterol (HDL-C)levels,and liver TG,TC levels were determined,as well as SIRT1 and LXRα protein levels were measured.ResultsCompared with the control group,the serum TG,TC,and LDL-C levels were 1.25±0.25,4.29±0.59,and 1.59±0.20 mmol/L;liver TG(5.78±1.69 mmol/L)and TC(2.69±1.20 mmol/L)levels in the mice of high fat group were increased,and liver LXRα(0.36±0.03)and SIRT1(0.78±0.07)were decreased(P<0.05).Compared with the high-fat group,serum TG(0.91±0.15 mmol/L),TC(3.65±0.36 mmol/L)and LDL-C(1.39±0.11)in the mice of RSV intervention group and liver TG(4.63±1.70 mmol/L)and TC (1.65±0.89 mmol/L)were decreased significantly,but liver SIRT1(0.41±0.05)and LXRα(0.88± 0.09)increased significantly (P<0.05).ConclusionResveratrol intervention could effectively improve abnormal lipid metabolism in C57BL/6J mice with high fat diet,which may be related to the promotion of liver LXRα and SIRT1 expression.