地沟油标准物质候选物研制

目的 为制备“地沟油”标准物质候选物探讨和选择适当原料、工艺路线和质控指标。方法 选择“地沟油”目前最常用和最易得的原料、最有可能采用的精炼工艺路线,以外观、气滋味、色泽、水分及挥发物、酸价为质控指标,制备“地沟油”标准物质候选物。结果 选择餐厨废弃油脂为原料,通过蒸馏脱臭-碱炼脱酸-吸附脱色制备“地沟油”标准物质候选物,各批次候选物的色泽（1”,R）在4.0~5.1,水分及挥发物在0.02%~0.05%,酸价在5.60 ~6.30 mg/g。结论 利用该候选物可制备“地沟油”特定内源性成分和外源性成分标准物质或测试参比样品,可全面寻找其中特定的内源性和外源性标志物,验证选定标志物的特异性、灵敏度和检出限。

Preparation of reference material for detection of bio-waste oil

Objective To study candidate reference materials,processes route and quality control indexes for the detection of bio-waste oil.Methods Using the most common and convenient raw material and the most probable technical processes,reference material for bio-waste oil detection was screened taking the appearances,gas taste,color,water and volatiles,acid value as quality control indexes.Results Kitchen waste oils were collected and candidates of bio-waste oil reference materials were prepared by the processes of distilling deodorization,alkali refining deacidification and adsorption decolorization.These candidates have the index values of color(1",R)between 4.0-5.1,water and volatile between 0.02%-0.05%,and acid between 5.60-6.30 mg/g.Conclusion The candidates could be used to prepare reference material for detection of specific substance in bio-waste oils with good specificity,sensitivity and detection limit.