Comparison of Restriction Fragment Length Polymorphism, Microsatellite Length Polymorphism, and Random Amplification of Polymorphic DNA Analyses for Fingerprinting Aspergillus fumigatus Isolates |
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| Authors: | Emmanuelle Bart-Delabesse Jacqueline Sarfati Jean-Paul Debeaupuis Willem van Leeuwen Alex van Belkum Stephane Bretagne Jean-Paul Latge |
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| Affiliation: | Laboratoire de Parasitologie-Mycologie, H?pital Henri Mondor, Créteil, France. |
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| Abstract: | Aspergillus fumigatus fingerprints generated by random amplification of polymorphic DNA (RAPD), restriction fragment length polymorphism (RFLP) upon hybridization with repeated DNA sequences, and PCR detection of microsatellite length polymorphism (MLP) were compared among 67 isolates. In contrast to RAPD, RFLP and MLP gave discriminating and significantly concordant genotyping results. |
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