巨噬细胞加工与呈递日本血吸虫抗原的作用

以免疫印迹技术探讨Mφ对日本血吸虫UEA(卵尿素溶性抗原)的加工情况。检测对象为UEA体外激活的Mφ匀浆及Mφ培养上清。所用结合物为辣根过氧化物酶标记的经亲和层析提纯的小鼠抗UEA抗体。结果可见抗原经Mφ作用后,大分子量UEA形成小分子多肽。它们与UEA及胰酶酶解的UEA有明显差异;培养上清与细胞匀浆条带较相似,仅见某些量的不同。根据以上结果而推论:(1)UEA可被Mφ加工为小分子片段,加工方式为裂解;(2)加工后的抗原多肽可释出Mφ外。

PRELIMINARY STUDY ON THE ROLE OF MACROPHAGE IN PROCESSING AND PRESENTING ANTIGENS OF SCHISTOSOMA JAPONICUM

The horseradish peroxidase labeled affinity purified mice anti-UEA (Urea soluble egg antigen of Schistosoma japonicum) antibody was used in enzyme-linked immunoelectrotransfer blot (EITB) to monitor the changes after UEA being processed by M phi. The immune responsive peptides were detected in the culture supernatant and homogenate of M phi pulsed with UEA in vitro (M phi+). After processing by M phi the high molecular weight UEA was cleaved into low molecular weight peptides, as shown, by the reactive bands. They markedly differed from that native UEA or trypsin-digested UEA. The bands of M phi supernatant and homogenate showed similarity with certain quantitative differences. According to the result described above, we considered: 1. UEA could be processed into smaller pieces by M phi, the style of processing is cleavage. 2. The processed peptides might be released to extracellular environment.