| 大枣多糖的分离、纯化及分子量的测定 |
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| 引用本文： | 杨云,李振国,孟江,苗明三,魏红. 大枣多糖的分离、纯化及分子量的测定[J]. 世界科学技术, 2003, 5(3): 53-55 |
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| 作者姓名： | 杨云 李振国 孟江 苗明三 魏红 |
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| 作者单位： | [1]河南中医学院郑州450008 [2]河南省药检所郑州450008 [3]河南省商丘市产品质量监督检验所商丘476000 |
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| 基金项目： | 河南重大科技攻关项目(984021200) |
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| 摘 要： | 大枣果肉用乙醇回流脱脂，80～100℃热水浸提，提取液经减压浓缩，乙醇分级沉淀，三氯乙酸脱蛋白，透析，透析内液再经减压浓缩、醇沉得到三个粗多糖ZJ—A’，ZJ—B’，ZJ—C’，ZJ—B’经DEAE—Cellulose柱、Sephacryl S—300柱反复纯化，得大枣多糖ZJ—1、ZJ—2，高效凝胶渗透色谱(HPGPC)和常压凝胶柱色谱分析表明，ZJ—1、ZJ—2为均一多糖，HPGPC法测定分子量(MW)分别为9848、8991，紫外吸收光谱表明，ZJ—1、ZJ—2无核酸和蛋白质的特征吸收峰。
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| 关 键 词： | 大枣多糖 分子量 测定 分离 纯化 植物药 |
| 修稿时间： | 2002-12-20 |
Isolation and Purification of Polysaccharides in Zizyphus Jujuba MILL and Determination of their Molecular weight |
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| Yang Yun Meng Jiang and Miao Mingsan. Isolation and Purification of Polysaccharides in Zizyphus Jujuba MILL and Determination of their Molecular weight[J]. World Science and Technology(Modernization of Traditional Chinese Medicine), 2003, 5(3): 53-55 |
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| Authors: | Yang Yun Meng Jiang and Miao Mingsan |
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| Abstract: | The flesh of Zizyphus jujube MILL is defatted by the way of ethanol reflux and extracted in 80-1000C water; the extracted liquid is concentrated by reducing its pressure, and the fractions ZJ-A, ZJ-B and ZJ-C are obtained by fractional precipitation of the liquid with ethanol and clearing away protein from it by CC13COOH. As the result, ZJ-land ZJ-2 are obtained via further purification of ZJ-B and their DEAE -Cellulose and Sephacryl S-300 column chromatography, and their homogeneity is verified by HPGPC and Sephacry S-300. No absorption peaks of protein and nucleic acid are found from Zj-1 and Zj-2 by UV-scan and the molecular weights of Zj-1 and Zj-2 are 9848 and 8991 respectively by the determination of HPGPC. |
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| Keywords: | Zizyphus jujube MILL polysaccharide isolation purification determination of molecular weight |
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