Prevalence of virulence genes and cytolethal distending toxin production in Campylobacter jejuni isolates from diarrheal patients in Bangladesh |
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| Authors: | Talukder Kaisar A Aslam Mohammad Islam Zhahirul Azmi Ishrat J Dutta Dilip K Hossain Sabir Nur-E-Kamal Alam Nair Gopinath B Cravioto Alejandro Sack David A Endtz Hubert P |
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| Affiliation: | Enteric Microbiology Laboratory, Laboratory Sciences Division, ICDDR,B, GPO Box 128, Dhaka 1000, Bangladesh. kaisar@icddrb.org |
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| Abstract: | From 300 stool samples, 58 Campylobacter strains were isolated by standard microbiological and biochemical methods. Of these, 40 strains were identified as Campylobacter jejuni and 5 as Campylobacter coli. The presence of flaA (100%), cadF (100%), racR (100%), dnaJ (100%), pldA (100%), ciaB (95%), virB11 (0%), ceuE (82.5%), cdtA (97.5%), cdtB (97.5%), cdtC (97.5%), and wlaN (7.5%) genes was detected in C. jejuni by PCR. All C. jejuni strains but one produced cytolethal distending toxin in a HeLa cell assay. |
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