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| 三肽化合物酪丝缬肽对小鼠黑色素瘤细胞B16-F10侵袭和转移的抑制作用 | |
| 引用本文： | Che XC,Lu R,Hu JX,Zheng MN,Zhang MF,Wang S,Yu CY,Yang XL,Xing DH,Yao Z. 三肽化合物酪丝缬肽对小鼠黑色素瘤细胞B16-F10侵袭和转移的抑制作用[J]. 癌症, 2006, 25(3): 275-280 |
| 作者姓名： | Che XC Lu R Hu JX Zheng MN Zhang MF Wang S Yu CY Yang XL Xing DH Yao Z |
| 作者单位： | 1. 天津医科大学免疫教研室,天津,300070 2. 天津医科大学免疫教研室,天津,300070;深圳康哲药业有限公司,广东,深圳,518029 3. 天津医科大学病理教研室,天津,300070 |
| 基金项目： | 国家科技攻关项目;国家重点基础研究发展基金(973计划);全国教育科学规划项目 |
| 摘 要： | 背景与目的:三肽化合物酪丝缬肽(tyroservaltide,YSV)对实验性肝癌具有明显抑制作用,本研究观察YSV对小鼠黑色素瘤细胞B16-F10侵袭和转移的抑制作用。方法:MTT法检测YSV对B16-F10的细胞毒作用;利用基质胶Matrigel研究YSV对细胞粘附能力的影响;用Transwell小室侵袭模型研究YSV对肿瘤细胞侵袭能力的改变;经C57/BL6小鼠尾静脉注射B16-F10细胞,建立人工肺转移模型,观察YSV对B16-F10肺转移的影响;免疫组化方法观察YSV对细胞间粘附分子(intercellularadhesionmolecule-1,ICAM-1)在肺组织中表达水平的影响。结果:100μg/mlYSV作用48h对B16-F10细胞的增殖抑制率为24.36%;作用24h对B16-F10细胞在Matrigel胶上的粘附抑制率为36.51%;10μg/mlYSV作用48h对B16-F10细胞的侵袭抑制率为36.53%;640μg·(kg·d)-1YSV抑制B16-F10的肺转移,抑制率为62.21%;YSV组ICAM-1的组织量明显少于生理盐水组。结论:YSV具有抑制B16-F10生长和侵袭转移的作用。 |
| 关 键 词： | 酪丝缬肽 B16-F10细胞 侵袭 转移 小鼠 |
| 文章编号： | 1000-467X（2006）03-0275-06 |
| 收稿时间： | 2005-06-21 |
| 修稿时间： | 2005-08-22 |
Inhibitory effect of tripeptide compound tyroservaltide on invasion and metastasis of mouse melanoma cell line B16-F10 |
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| Che Xu-Chun,Lu Rong,Hu Jing-Xia,Zheng Min-Na,Zhang Ming-Fang,Wang Song,Yu Chun-Yan,Yang Xiang-Long,Xing Dong-Hong,Yao Zhi. Inhibitory effect of tripeptide compound tyroservaltide on invasion and metastasis of mouse melanoma cell line B16-F10[J]. Chinese journal of cancer, 2006, 25(3): 275-280 | |
| Authors: | Che Xu-Chun Lu Rong Hu Jing-Xia Zheng Min-Na Zhang Ming-Fang Wang Song Yu Chun-Yan Yang Xiang-Long Xing Dong-Hong Yao Zhi |
| Affiliation: | Department of Immunology, Tianjin Medical University, Tianjin 300070, P. R. China. |
| Abstract: | BACKGROUND & OBJECTIVE: Tripeptide compound tyroservaltide (YSV) has obvious inhibitory effect on experimental liver cancer. This study was to evaluate the inhibitory effect of YSV on the invasion and metastasis of mouse melanoma cell line B16-F10. METHODS: The cytotoxic effect of YSV on B16-F10 cells was assessed by MTT assay, and the effects of YSV on the adhesiveness and invasiveness of B16-F10 cells were determined using Matrigel and a transwell system. B16-F10 cells were injected into the tail veins of C57BL/6 mice to establish an experimental lung metastasis model, and the effect of YSV on lung metastasis was observed. The effect of YSV on the expression of intercellular adhesion molecule-1 (ICAM-1) in lung tissue was detected by immunohistochemistry. RESULTS: YSV (100 microg/ml, 48 h) inhibited the proliferation of B16-F10 cells, with an inhibitory rate of 24.36%; YSV (100 microg/ml, 24 h) inhibited the adhesiveness of B16-F10 cells to Matrigel, with an inhibitory rate of 36.51%; YSV (10 microg/ml, 48 h) inhibited the invasiveness of B16-F10 cells, with an inhibitory rate of 36.53%; YSV [640 microg.(kg.d)-1] inhibited lung metastasis by B16-F10 cells, with an inhibitory rate of 62.21%. The expression of ICAM-1 in lung tissue was lower in YSV group than in normal saline group. CONCLUSION: YSV can inhibit the growth, invasion, and metastasis of B16-F10 cells. |
| Keywords: | Tyroservaltide B16-F10 cell Invasion Metastasis Mouse |
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