| 正常人血清中IgG类抗角蛋白自身抗体的免疫印迹分析 |
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| 引用本文： | 兰风华 刘玉峰. 正常人血清中IgG类抗角蛋白自身抗体的免疫印迹分析[J]. 现代免疫学, 1999, 19(5): 294-295 |
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| 作者姓名： | 兰风华 刘玉峰 |
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| 作者单位： | 南京军区福州总医院!福州350025(兰风华),西京医院皮肤科!西安710032(刘玉峰),第四军医大学生物化学与分子生物学教研室!西安710032(陈苏民) |
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| 基金项目： | 国家新药（1035 课题） 基金 |
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| 摘 要： | 抗角蛋白自身抗体是正常人天然自身抗体的一部分。本研究用一组角蛋白对正常人血清中的ＩｇＧ 类抗角蛋白抗体进行了免疫印迹分析。２１ 份正常人血清均可识别至少一种角蛋白成分, 可区分出１４ 种反应性模式。３ 份合并血清的反应性模式高度均一。结果表明, 血清抗角蛋白抗体普遍存在, 但在人群中呈高度异质性; 不同个体血清可互补。角蛋白 抗角蛋白系统可能是天然自身抗体研究的一个理想模式系统。
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| 关 键 词： | 抗角蛋白自身抗体 天然自身抗体 角蛋白 免疫印迹 |
Western Blot Analysis of Antikeratin Autoantibodies in Normal Human Sera |
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| Lan Fenghua ,Liu Yufeng ,Chen Sumin. Western Blot Analysis of Antikeratin Autoantibodies in Normal Human Sera[J]. Current Immunology, 1999, 19(5): 294-295 |
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| Authors: | Lan Fenghua Liu Yufeng Chen Sumin |
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| Affiliation: | Lan Fenghua 1,Liu Yufeng 2,Chen Sumin 1 |
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| Abstract: | To get insights into the immunochemical properties of natural antikeratin autoantibodies,which constitute part of the natural autoantibody pool,Western blot analysis of IgG antikeratin autoantibodies in normal human sera was carried out using a group of keratins Each of the 21 sera tested could react to at least one keratin component,and up to 14 reactivity patterns were identified The reactivity patterns of 3 pooled sera were highly homogenous The results indicate that the antikeratin autoantibodies are generally present in normal humans,but the reactivity patterns are highly heterogeneous Individual sera are complimentary in reactivity The natural keratin antikeratin system would be an ideal model for the study of natural autoantibodies |
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| Keywords: | antikeratin autoantibodies natural autoantibodies keratin Western blotting |
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