慢性乙型肝炎患者血清瘦素和外周血B细胞、T细胞亚群测定的临床意义

目的探讨血清瘦素表达水平和外周血B细胞、T细胞亚群在慢性乙型肝炎病毒感染造成肝功能损伤过程中的作用。方法分别采用酶联免疫吸附试验（ELISA）和单克隆抗体法检测36例慢性乙型肝炎患者和30例健康体检者血清瘦素水平和外周血B细胞、T细胞亚群水平,并检测两组肝功能指标。结果慢性乙型肝炎患者瘦素、B细胞数均非常显著地高于正常人组（P〈0.01）,而CD3、CD4、CD4/CD8则显著地低于正常人组（P〈0.01）,且与ALT、TBil水平呈明显的正相关。结论检测慢性乙型肝炎患者血清瘦素水平以及外周血B细胞和T细胞亚群水平的变化在一定程度上反映了机体的免疫功能状态和肝脏的受损程度,具有一定的临床实用价值。

The detection and clinical significance of serum leptin,peripheral B cell number,T cell subsets distribution type in patients with chronic hepatitis B

Objective To investigate the changes of serum leptin level,B cell number,T-cell subsets in patients with chronic hepatitis B and the relationship between the changes and liver function alterations.Methods Serum leptin levels（with RIA）,peripheral B cell number,T cell subsets distribution type（with monoclonal antibody technique）,ALT and total bilirubin（with biochemistry）were measured in 36 patients with chronic hepatitis B and 30 controls.Results The serum leptin lever and B cell percentage in the patients were significantly higher than those in controls（P〈0.01）,while the CD3,CD4 percentage and CD4/CD8 ratio were significantly lower（P〈0.01）.The serum leptin levels were significantly positively correlated with those ALT and total bilirubin（P〈0.01）.Conclusions Serum leptin levels correlated well with those of two major liver function indicators（ALT and total bilirubin）,thus,leptin might be used as a part of liver function test battery.Changes in B and T cell subsets demonstrates immuno-disturbances in patients with chronic hepatitis B.