骨髓基质细胞在脂多糖诱导的肺损伤小鼠肺组织的定植研究

目的：探讨静脉输注骨髓基质细胞（BMSCs）在脂多糖（LPS）诱导的肺损伤小鼠肺组织定植并向肺泡上皮细胞分化的可能性。方法：绿色荧光蛋白（GFP）转基因C57BL/6J小鼠作为BMSCs移植供体，同种野生型小鼠为移植受体。气道滴入LPS诱导肺损伤。受体分为以下几组：(1) PBS+BMSCs移植（CM）; (2) LPS+BMSCs 移植（LM）; (3) PBS+全身放射+BMSCs移植（CIM）; (4) LPS+全身放射+BMSCs移植（LIM）。移植14 d后，以免疫荧光双标染色检测BMSCs在受体肺组织的定植情况，流式细胞仪检测体外培养的肺泡II型上皮细胞（AEC II）GFP阳性率，荧光定量PCR法检测肺组织表面活性物质蛋白（SP）-A、SP-C和水通道蛋白（AQP）-5 mRNA的表达。结果：移植后14 d，免疫荧光双标染色可见CIM和LIM组有少量肺泡上皮细胞呈GFP和角蛋白双染阳性，而且LIM组较CIM组有较多阳性细胞。与CM和LM组（分别为2.82±1.03%和3.81±0.93%）比较，CIM和LIM组的AEC II GFP阳性率更高（分别为11.10±3.19% 和14.40±2.40%; P<0.05）。LIM组SP-C mRNA表达较CM组增高(2.09±0.18 vs 1.38±0.30；P<0.05)。结论：供体来源的BMSCs能在LPS诱导的受损肺组织定植分化，提示静脉输注BMSCs可能有助于肺损伤的修复。［中国当代儿科杂志，2009，11（5）：321-327］

Engraftment of bone marrow stromal cells in lipopolysaccharide-injured mouse lungs

Objective To explore a feasibility of engraftment of systemically transplanted bone marrow stromal cells (BMSCs) and differentiation into lung epithelial cells in lipopolysaccharides (LPS)-injured lungs.Methods BMSCs were isolated from bone marrow of transgenic green fluorescent protein (GFP) C57BL/6J mice and systemically administered to bone marrow-suppressed wild-type C57BL/6J mice. A mouse model of lung injury was prepared by intratracheal instillation of LPS. Recipients were assigned to four groups:int...