Expression and localization of Smadl, Smad2 and Smad4 proteins in rat testis during postnatal development

Aim: To study the expression and regulation of Smadl, Smad2 and Smad4 proteins (intracellular signaling molecules of transforming growth factor-β family) in rat testis during postnatal development. Methods: The whole testes were collected from SD rats aged 3, 7, 14, 28 and 90 (adult) days. The cellular localization and developmental changes were examined by immunohistochemistry ABC method with the glucose oxidase-DAB-nickel enhancement technique. Quantitative analysis of the immunostaining was made by the image analysis system. The Smads proteins coexistence in the adult rat testis was tested by the double immune staining for CD14-Smad4 and Smad2-Smad4. The protein expression of Smad during rat testicular development was examined by means of Western blots. Results: Smadl, Smad2 and Smad4 were present throughout testicular development. The immunostaining of Smadl and Smad2 were present in spermatogenic cells. A positive immunoreactivity was located at the cytoplasm, but the nucleus was negative. Smadl wa

Expression and localization of Smadl, Smad2 and Smad4 proteins in rat testis during postnatal development</b></span></td> </tr> <tr> <td align="left" height="15" valign="top"></td><td valign="top" align="left"></td> </tr> <tr> <td align="left" height="25" valign="top" colspan=2"><author>Jing HU,Yuan-Qiang ZHANG,Xin-Ping LIU,Rui-An WANG,Yan JIN,Ruo-Jun XU. <title>Expression and localization of Smadl, Smad2 and Smad4 proteins in rat testis during postnatal development[J]. Asian journal of andrology, 2003, 0(1)</td> </tr> <tr> <td align="left" height="10" valign="top"></td><td valign="top" align="left"></td> </tr> <tr> <td align="left" height="25" valign="top" nowrap><b>Authors:</b></td><td width="92%" valign="top" align="left" ><a target='_blank' href='search.aspx?subject=medicine_health&major=wkx&orderby=referenced&field=author_name&q=%3cauthor%3eJing+HU&prev_q=%3cauthor%3eJing+HU'><author>Jing HU</a> <a target='_blank' href='search.aspx?subject=medicine_health&major=wkx&orderby=referenced&field=author_name&q=Yuan-Qiang+ZHANG&prev_q=Yuan-Qiang+ZHANG'>Yuan-Qiang ZHANG</a> <a target='_blank' href='search.aspx?subject=medicine_health&major=wkx&orderby=referenced&field=author_name&q=Xin-Ping+LIU&prev_q=Xin-Ping+LIU'>Xin-Ping LIU</a> <a target='_blank' href='search.aspx?subject=medicine_health&major=wkx&orderby=referenced&field=author_name&q=Rui-An+WANG&prev_q=Rui-An+WANG'>Rui-An WANG</a> <a target='_blank' href='search.aspx?subject=medicine_health&major=wkx&orderby=referenced&field=author_name&q=Yan+JIN&prev_q=Yan+JIN'>Yan JIN</a> <a target='_blank' href='search.aspx?subject=medicine_health&major=wkx&orderby=referenced&field=author_name&q=Ruo-Jun+XU&prev_q=Ruo-Jun+XU'>Ruo-Jun XU</a></td> </tr> <tr><td align="left" height="10" valign="top"></td><td valign="top" align="left"></td></tr> <tr> <td align="left" height="25" valign="top" nowrap><b>Affiliation:</b></td><td width="92%" valign="top" align="left" ><author>Jing HU,Yuan-Qiang ZHANG,Xin-Ping LIU,Rui-An WANG,Yan JIN,Ruo-Jun XUDepartment of Histology and Embryology,Department of Biochemistry and Molecular Biology,Department of OralHistology and Pathology,School of Dentistry,Fourth Military Medical University,Xi'an 710032,ChinaDepartment of Zoology,University of Hong Kong,Hong Kong,HKSAR,China</td> </tr> <tr><td align="left" height="10" valign="top"></td><td valign="top" align="left"></td></tr> <tr> <td align="left" height="25" valign="top" nowrap><b>Abstract:</b></td><td width="92%" align="left" valign="top"></td> </tr> <tr> <td align="left" height="15" valign="top"></td><td valign="top" align="left"></td> </tr> <tr> <td align="left" valign="top" height="25" nowrap><b>Keywords:</b></td><td width="92%" valign="top" align="left" ></td> </tr> <tr> <td align="left" colspan="2" height="30"><b>本文献已被 <a href="http://www.cnki.com.cn/Article/CJFDTotal-YZNK200301014.htm" target="_blank">CNKI</a> 等数据库收录！</b></td> </tr> </table> </td> </tr> </table> <table width="870" align="center"> <tr><td align="left"> <div id="WasReferencedList"> </div> </td></tr> <tr><td align="left"> <div id="RelateArticleList"> </div> </td></tr> </table> <table width="870" border="0" align="center" cellpadding="0" cellspacing="0"> <tr> <td height="40" align="center" class="fo2"> <a href="#" onClick="this.style.behavior='url(#default#homepage)';this.setHomePage('http://yyws.alljournals.cn')">设为首页</a> <span class="STYLE1">|</span> <a href="http://www.alljournals.cn/note.aspx">免责声明</a> <span class="STYLE1">|</span> <a href="http://www.e-tiller.com"><u>关于勤云</u></a> <span class="STYLE1">|</span> <a href="javascript:window.external.addFavorite('http://yyws.alljournals.cn','期刊界 All Journals---医药、卫生')">加入收藏</a></td> </tr> <tr> <td align="center" class="fo3"> <p align="center"> Copyright<span lang="en">©<a href="http://www.e-tiller.com"><u>北京勤云科技发展有限公司</u></a> 京ICP备09084417号 </span></p> </td> </tr> </table> </body> </html> <script language=javascript> $(document).ready(function() { $("div#WasReferencedList").html("正在获取引用信息，请稍候..."); $.get("was_referenced_list.aspx?pcid=A9DB1C13C87CE289EA38239A9433C9DC&cid=527A01A248DACB72&jid=A992D1656D1AB4B4D7B77D105E0C5A20&yid=D43C4A19B2EE3C0A&aid=5026A88B135621BD705A2E2F32467A01&iid=CA4FD0336C81A37A&is_more=0&cp=1&tn=0",function(xml){ if(xml==""){ $("div#WasReferencedList").html(""); }else{ $("div#WasReferencedList").html(xml); } }); $("div#RelateArticleList").html("正在获取相似文献，请稍候..."); $.get("relate_article_list.aspx?pcid=A9DB1C13C87CE289EA38239A9433C9DC&cid=527A01A248DACB72&aid=5026A88B135621BD705A2E2F32467A01&language=2&ctl=105&etl=112",function(xml){ if(xml==""){ $("div#RelateArticleList").html(""); }else{ $("div#RelateArticleList").html(xml); } }); }); </script>