| 中国仓鼠细胞CTP合成酶cDNA的克隆及测序 |
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| 引用本文： | 翟朝阳.中国仓鼠细胞CTP合成酶cDNA的克隆及测序[J].四川大学学报(医学版),1995(1). |
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| 作者姓名： | 翟朝阳 |
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| 作者单位： | 1. Laboratory of Molecular Biology;2. Department of Biology，Hong Kong University of Science and Technology;3. Department of Human Genetics，University of Michigan Medical School |
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| 摘 要： | 用ＲＡＣＥ－ＰＣＲ方法合成了中国仓鼠细胞ＣＴＰ合成酶的ｃＤＮＡ，经克隆到载体并转化大肠杆菌。从大小不同的插入片段中筛选出一个１．３ｋｂ的片段进行测序。结果表明，此片段为ＣＴＰ合成酶的ＣＤＮＡ。
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| 关 键 词： | CTP合成酶，cDNA，中国仓鼠细胞 |
Cloning and Sequencing of CTP Synthetase cDNA from Chinese Hamster Cells |
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| Zhai Chaoyang, Mette T Skaanild, Ernest HY Chu.Cloning and Sequencing of CTP Synthetase cDNA from Chinese Hamster Cells[J].Journal of West China University of Medical Sciences,1995(1). |
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| Authors: | Zhai Chaoyang Mette T Skaanild Ernest HY Chu |
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| Abstract: | The CTP synthetase cDNA was clonedfrom Chinese hamster lung cells V_(79) .By comparison ofthe amino acid sequence of CTP synthetase with thecounterpart of other species published,it was showthat the CTP synthetase was a highly conserved andstable gene among the mammalian cells, but more dif-ferences of the CTP synthetase sequence could be seenbetween procaryotic and eukaryotic cells.This phe-nomenon suggested that the catalysing model of theenzyme might have some small differences in the or-ganisms. |
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| Keywords: | CTP synthetase cDNA Chinese hamster cells |
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