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| 壳聚糖纳米粒介导人表皮生长因子受体2小干扰RNA抑制乳腺癌细胞迁移 | |
| 引用本文： | 张永成,武晓英,陈晓兰. 壳聚糖纳米粒介导人表皮生长因子受体2小干扰RNA抑制乳腺癌细胞迁移[J]. 中华乳腺病杂志(电子版), 2011, 5(5): 36-40 |
| 作者姓名： | 张永成 武晓英 陈晓兰 |
| 作者单位： | 1. 广东药学院附属第一医院乳腺科,广州,510080 2. 广东药学院基础学院生物学教研室,广州,510006 |
| 摘 要： | 目的以壳聚糖纳米粒作为人类表皮生长因子受体2小干扰RNA（HER-2siRNA）的载体转染人乳腺癌细胞SK—BR-3，观察HER-2沉默对其体外迁移能力的影响。方法采用离子凝胶法制备壳聚糖．HER．2siRNA纳米粒，原子力显微镜观察纳米粒的形态。分4组进行转染，分别加入转染试剂壳聚糖-HER-2siRNA纳米粒（实验组）、Lipofectamine^TM2000和HER-2siRNA（阳性对照组）、壳聚糖-阴性对照siRNA纳米粒（阴性对照组）和空白壳聚糖纳米粒（空白组）。-步法逆转录-聚合酶链反应（RT—PCR）和Westernblot鉴定HER-2在SK—BR-3细胞中的表达变化。Transwell小室法检测HER-2siRNA对细胞体外迁移能力的影响。统计分析采用单因素方差分析、K—WH秩和检验。结果成功制备了壳聚糖-siRNA纳米粒，呈球形，大小约100nm。实验组能有效抑制HER-2mRNA和蛋白的表达以及SK—BR-3细胞的体外迁移能力，与阴性对照组和空白组相比差异有显著的统计学意义（P均=0．000）。结论壳聚糖纳米粒介导的HER-2siRNA能有效抑制人乳腺癌细胞SK—BR-3中HER-2的表达及其体外迁移能力。 |
| 关 键 词： | 乳腺肿瘤 壳聚糖纳米粒 HER-2 RNA干扰 迁移 |
Inhibitory effect of chitosan nanoparticles-mediated HER-2 siRNA on migration of breast cancer cell line SK-BR-3 |
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| ZHANG Yong-cheng,WU Xiao-ying,CHEN Xiao-lan. Inhibitory effect of chitosan nanoparticles-mediated HER-2 siRNA on migration of breast cancer cell line SK-BR-3[J]. Chinese Journal of Breast Disease(Electronic Version), 2011, 5(5): 36-40 | |
| Authors: | ZHANG Yong-cheng WU Xiao-ying CHEN Xiao-lan |
| Affiliation: | ZHANG Yong-cheng,WU Xiao-ying,CHEN Xiao-lan.Department of Breast Surgery,First Affiliated Hospital of Guangdong Pharmaceutical University,Guangzhou 510080,China |
| Abstract: | ObjectiveTo investigate the effect of chitosan nanoparticles loaded with small interference RNA(siRNA) of HER-2(human epidermal growth factor receptor 2)on in vitro migratory activity of SK-BR-3 cells from a human breast carcinoma cell line.MethodsChitosan-siRNA nanoparticle targeting HER-2 mRNA was prepared by ionic gelation using tripolyphosphate,and the morphology of the nanoparticles was observed with atomic force microscope.The transfected SK-BR-3 cells were divided into 4 groups:the experimental group... |
| Keywords: | breast neoplasms chitosan nanoparticles HER-2 RNA interference migration |
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