| 盐酸帕洛诺司琼注射液中异构体含量的NP-HPLC测定 |
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| 引用本文： | 潘红娟,王哲峰,时惠麟,俞雄.盐酸帕洛诺司琼注射液中异构体含量的NP-HPLC测定[J].中国医药工业杂志,2009,40(8). |
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| 作者姓名： | 潘红娟 王哲峰 时惠麟 俞雄 |
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| 作者单位： | 1. 上海医药工业研究院;创新药物与制药工艺国家重点实验室,上海,200437
2. 上海医药工业研究院 |
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| 摘 要： | 建立了正相高效液相色谱(NP-HPLC)法测定盐酸帕洛诺司琼注射液中(R,S)-异构体的含量.样品经氧氧化钠碱化后,再用二氯甲烷提取.用Inertsil SIL-100A色谱柱,二氯甲烷-甲醇-氨水(448:50:2)为流动相,检测波长261 nm.采用加校正因子的主成分自身对照法计算(R,S)-异构体的含量.
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| 关 键 词： | 盐酸帕洛诺司琼 异构体 正相高效液相色谱 测定 |
Determination of (R,S)-Isomer in Palonosetron Hydrochloride Injection by NP-HPLC |
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| Authors: | PAN Hongjuan WANG Zhefeng SHI Huilin YU Xiong |
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| Abstract: | A NP-HPLC method was established for the determination of (R,S)-isomer in palonosetron hydrochloride injection. The samples was treated by NaOH, and then extracted with dichloromethane. An Inertsil SIL-100A column was used, with the mobile phase of dichloromethane-methanol-ammonia solution (448 : 50 : 2), at the detection wavelength of 261 nm. The content of (R,S) - isomer was calculated based on palonosetron as the reference solution calibrated with correction factors. |
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| Keywords: | palonosetron hydrochloride isomer NP-HPLC determination |
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