雌、孕激素受体和基质金属蛋白酶-2在子宫腺肌症中的表达及意义

目的探讨雌激素受体（ER）、孕激素受体（PR）及基质金属蛋白酶-2（MMP-2）在子宫腺肌病（UAM）中的表达和意义。方法应用免疫组化二步法检测35例UAM患者的异位内膜、在位内膜及38例非UAM患者正常内膜中ER、PR和MMP-2的表达情况,通过统计学方法行对照研究。结果 （1）UAM异位内膜组织中ER、PR的表达较对照正常内膜低（P〈0.05）;（2）UAM异位内膜中MMP-2表达水平显著高于正常内膜组（P〈0.01）;（3）MMP-2蛋白在UAM异位内膜中表达有周期性变化,增生期高于分泌期（P〈0.05）,而在在位内膜及对照内膜中表达无明显周期性变化。结论 ER表达并非子宫腺肌病的主要致病因素;MMP-2在异位内膜的高表达可能使异位内膜具有更高的侵袭性,并且在增生期表达活跃。

EXPRESSION AND SIGNIFICANCE OF ER,PR AND MMP- 2 IN UTERINE ADENOMYOSIS

Objective To explore the expression and significance of ER, PR and MMP - 2 in Uterine Adenomy- osis （UAM）. Methods Immunohistochemical method was employed to detect the expression of estrogen（ER） ,pro- gesterone receptor（PR） and Matrix Mealloproteinase- 2（ MMP-2） in the ectopic endometrium and the eutopic en- dometrium of 35 patients with UAM and the normal controls（ n = 38 ） ,then the correlation of the expression of them was analyzed. Results （ 1 ） The expression of ER and PR in ectopic endometrium were significantly lower than that in normal endometrium （ P 〈 O. 05 ） ; （2） The expression of MMP - 2 was obviously higher in the ectopic endometri- um of UAM patients than that of controls（ P 〈0. O1 ） ;（3 ）The expression of MMP-2 protein in ectopic endometri- um showed cyclic change obviously, the expression level of MMP -2 in proliferate stage was significantly higher than that in protestation stage （P 〈 0.05 ）. But in eutopic endometrium and the normal controls showed no cyclic change. Conclusions The high expression of MMP - 2 in the ectopic endometrium suggests that the ectopic endome- trium with UAM may be more invasive and the expression was higher in proliferation stage.