化学修饰电极对雏鸡耳蜗神经元一氧化氮的定性检测

目的 比较雏鸡和哺乳动物耳蜗生理功能的异同。过去已有哺乳动物耳蜗及前庭神经元中一氧化氮合酶（ＮＯＳ）活动证据的报道，其所释放的一氧化氮（ＮＯ）在听觉和平衡活动中可能发挥重要作用。方法 用经修饰的碳和有、定性检测鸡离体耳神经元在不同激动剂的诱发下所释放的ＮＯ。根据电极所检测的电流变化的幅度和持续时间判断ＮＯ的释放。结果 由激动剂乙酰胆碱和Ｌ－精氨酸所诱发的ＮＯ释放反应幅度相似，但乙酰胆碱的高电流持续

Study on nitric oxide in isolated cochlear neuron of chicken with chemically modified carbon fiber microelectrode]

OBJECTIVE: Nitric oxide(NO) is involved in neural signaling that is important to the physiologic and pathophysiologic activities of cochlea and vestibule. This study is to compare physiologic function of mammalian cochlea with that of chicken cochlea. METHODS: Using chemically modified carbon fiber microelectrode to measure qualitatively NO which was released from isolated cochlear neuron cluster of chicken in response to certain agonists, such as ATP, L-Arg and ACh. The presence of NO release is indicated by elevation of amplitude and lasting time of microelectric current. RESULTS: The results demonstrated that No releases stimulated by ACh and L-Arg were similar in amplitude but the lasting time stimulated by ACh was shorter than that of L-Aeg. Response to ATP, however, differred apparently in a manner which the amplitude was larger than that of ACh and L-Arg, whereas the lasting time approximated to that of L-Arg but longer than that of ACh. Before application of agonists, preapplication of L-NNA, a NOS antagonist no current changes could be monitored. CONCLUSION: These data suggested that chicken cochlear ganglion can synthesize and release NO. The effective mechanism of the agonists and possible role of NO in the cochlea is discussed.