日本血吸虫编码抱雌沟蛋白保守区cDNA核酸疫苗的研究

目的　为研究血吸虫性别特异性表达基因的核酸免疫特性。方法　本研究将所克隆的编码日本血吸虫中国大陆株抱雌沟蛋白基因保守区cDNA片段克隆到真核表达载体 pcDNA3中 ,得到的重组质粒直接免疫昆明系小鼠 ,感染血吸虫尾蚴 ,7周后剖杀小鼠冲虫 ,进行成虫和虫卵计数。结果　其减虫率为 32 4 % ,肝脏减卵率为 4 6 9% ,与对照组比较差异显著。结论　初步显示血吸虫抱雌沟蛋白基因DNA疫苗具有一定的核酸免疫保护功能

Preliminary studies on DNA vaccine of cDNA encoding the conservative region of gynecophoral canal protein of Schistosoma japonicum(Chinese strain)

Aim In order to observe the protective efficence induced by vaccinating animals with the DNA vaccine of Sex specific expression gene of Schistosoma Methods A 868 bp cDNA fragment amplified by RT PCR from adult Schistosoma japonicum(Chinese strain) mRNA was cloned into the eukaryotic expression vector pcDNA3 and the recombinant eukaryotic expression vector pcDNA3 SjGCP1 was directly injected into Kuming mice intramuscularly 3 times with the interval of 5 weeks Both the vaccinated and control group of mice were challenged with 40 cercariae of Sj 5 weeks after last injection and perfused 7 weeks post challenge Results The worm and egg counting reduction got from vaccinated mice was 32 4% and 46 9% respectively Conclusion The result indicates that pcDNA3 SjGCP1 DNA vaccine induces the significant protection in animal against Schistosoma japonicum infection