维生素D受体基因FokI酶切位点与胃癌的影响因素研究

目的：探讨维生素D受体基因 Fok I酶切位点多态性与胃癌的影响因素。方法随机选取2013年5月—2015年5月该院就诊的维吾尔族胃癌患者143例(A组)及维吾尔族健康对照者147例(B组),采用多聚酶链反应-限制性酶切片段长度多态性(Polymerase Chain Reaction-Restriction Fragment Length Polymorphism,PCR-RFLP)方法测定维生素D受体基因Fok I酶切位点多态性,进行两组间比较。结果单因素Logistic回归分析显示：胃癌患者的f等位基因频率高于对照组(58.0%vs 47.3%,P<0.05)。多因素Logistic回归分析显示：带有f等位基因(Ff+ ff)的受试者表现出较高的患胃癌的风险(OR值2.87)。结论 VDR的FokI酶切位点多态性是胃癌易感性影响因素。 f等位基因可能是胃癌发生的危险因素之一,而F等位基因可能是胃癌的保护因素。

Effect of Vitamin D Receptor Gene Foki Restriction Sites and Gastric Factors

Objective Discussion of vitamin D receptor gene Fok I restriction site polymorphism influencing factors and gastric cancer. Methods 143 cases of Uygur patients with gastric cancer treated in our hospital from May 2013 to May 2015 were randomly selected as the group A, 147 cases of Uygur healthy people treated in our hospital at the same period were selected as the group B (control group ), the polymorphism of vitamin D acceptor gene FokI restriction enzyme cutting site was measured by Polymerase Chain Reaction-Restriction Fragment Length Polymorphism method,PCR-RFLP and com-pared between the two groups. Results The single factor Logistic regression analysis showed that F allele frequency in the gastric cancer group was higher than that in the control group (58.0% vs 47.3%,P<0.05), multi-factor Logistic regression analysis showed that the subjects with F allele(Ff + ff)had a higher risk of gastric cancer(OR value was 2.87). Conclusion VDR FokI restriction site polymorphisms are susceptibility factors in gastric cancer. F allele may be one of the risk factors of occurrence of gastric cancer, however, F allele may the protective factor of gastric cancer.