IL-17调控IL-6/IL-6R/JAK1/STAT3信号通路影响喉癌细胞的侵袭和转移

目的 研究白介素(IL)-17通过IL-6/1L-6R/JAK1/STAT3信号通路调控人源性高分化喉癌细胞(Hep-2)侵袭、转移.方法 以Hep-2细胞为研究对象,设IL-17刺激剂0、1、50、100 ng/ml浓度组,Westem blot法检测不同分组在IL-17的刺激下,Hep-2细胞中IL-6、IL-6R、JAK1、p-JAK1、STAT3、p-STAT3的表达变化.迁移、侵袭实验观察IL-17的刺激对Hep-2细胞侵袭、迁移的影响.结果 Western blot实验中,在IL-17刺激下,1、50、100 ng/ml浓度组较0 ng/ml浓度组Hep-2细胞的IL-6、IL-6R、p-JAK1、p-STAT3的表达明显增加(p＜0.01),50、100 ng/ml浓度组较1 ng/ml浓度组Hep-2细胞的IL-6、1L-6R、p-JAK1、p-STAT3的表达明显增加(P＜0.01),100 ng/ml浓度组较50 ng/ml浓度组Hep-2细胞的IL-6R、p-JAK1、p-STAT3的表达明显增加(P＜0.01);各组间JAK1、STAT3的表达无明显变化.侵袭与迁移实验显示IL-17可以促进Hep-2细胞的侵袭和迁移.结论 IL-17可能通过IL-6/IL-6R/JAK1/STAT3信号通路促进Hep-2细胞的侵袭和转移.

IL-17 regulates IL-6/IL-6R/JAK1/STAT3 signaling pathway in invasion and metastasis of laryngeal carcinoma

Objective To investigate the effect of interleukin (IL)-17 on the invasion and metastasis of Hep-2 cells through IL-6/IL-6R/JAK1/STAT3 signaling pathway.Methods The concentration of IL-17 stimulated agent was 0,1,50 and 100 ng/ml in 0,1,50,100 ng/ml concentration group.The expression of IL-6,IL-6R,JAK1,p-JAK1,STAT3 and p-STAT3 in Hep-2 cells were detected by Western blot.We observed the migration and invasion of laryngeal carcinoma cells stimulated by interleukin-17.Results The expression of IL-6,IL-6R,p-JAK1 and p-STAT3 in 1,50,100 ng/ml concentration group was significantly higher than that in the 0 ng/ml concentration group (P ＜ 0.01).The expression of IL-6R,p-JAK1 and p-STAT3 in 50,100 ng/ml concentration group was significantly higher than that in the 1 ng/ml concentration group (P ＜ 0.01).The expression of IL-6,IL-6R,p-JAK1 and p-STAT3 in 100 ng/ml concentration group was significantly higher than that in the 50 ng/ml concentration group (P ＜0.01).There was no significant change in the expression of JAK1 and STAT3 between the groups.In the invasion and migration experiments,IL-17 can promote the invasion and migration of Hep-2 cells.Conclusion IL-17 may promote the invasion and metastasis of Hep-2 cells through IL-6/IL-6R/JAK1/STAT3 signaling pathway.