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MMP-14和CD31-MVD在结直肠癌组织中的表达及临床意义
引用本文:马晋疆,李志刚,侯吉学,黄桂林. MMP-14和CD31-MVD在结直肠癌组织中的表达及临床意义[J]. 安徽医科大学学报, 2017, 52(11): 1694-1698. DOI: 10.19405/j.cnki.issn1000-1492.2017.11.025
作者姓名:马晋疆  李志刚  侯吉学  黄桂林
作者单位:石河子大学医学院,石河子,832002;石河子大学医学院第一附属医院胃肠乳腺外科,石河子,832002
基金项目:新疆生产建设兵团卫生科技计划
摘    要:目的 探讨基质金属蛋白酶-14(MMP-14)和CD31标记的微血管密度(CD31-MVD)在结肠癌组织中的表达及其与肿瘤的临床意义.方法 采用免疫组织化学方法检测60例结直肠癌患者的癌组织及20例正常切缘组织中MMP-14和CD31-MVD的表达.采用qRT-PCR的方法检测20例新鲜结直肠癌组织及正常切缘组织中MMP-14 mRNA的相对表达量.结果 ①结直肠癌组织中的MMP-14阳性表达率以及CD3 1-MVD值明显高于正常切缘组织(P<0.05);②MMP-14的表达及CD31-MVD值与肿瘤大小、浸润深度、TNM分期和淋巴结转移有相关性(P<0.05),而与患者性别、年龄无明显相关;③MMP-14的表达与CD31-MVD呈正相关性(P<0.05);④结直肠癌组织中MMP-14 mRNA的相对表达量显著高于正常切缘组织(P<0.01).结论 结直肠癌组织中MMP-14的高表达和CD31标记的肿瘤组织微血管密度与结直肠癌的发生、浸润、转移有密切关系.

关 键 词:结直肠癌  基质金属蛋白酶-14  CD31-MVD  免疫组化  qRT-PCR

Expression of MMP-14 and CD31-MVD in colorectal carcinoma and its clinical significance
Abstract:Objective To investigate the expression of matrix metalloproteinase-14 (MMP-14) and CD31-MVD in colorectal carcinoma and their clinical significance.Methods The expression of MMP-14 and CD31-MVD were detected by immunohistochemistry in 60 cases of colorectal carcinoma and 20 cases of normal tissues from resection margin.The expression of MMP-14 mRNA were detected by qRT-PCR in 20 cases of fresh colorectal carcinoma tissues and normal tissues from resection margin.Results ① The expression of MMP-14 and CD31-MVD in colorectal carcinoma tissues were significantly higher than those in the normal tissues from resection margin (P < 0.05).② The expression of MMP-14 and CD31-MVD were correlated with tumor size,depth of invasion,TNM stage and lymph node metastasis (P < 0.05),but there was no significant correlation with gender and age of patients.③ The expression of MMP-14 was positively correlated with CD31-MVD.④ The expression of MMP-14 mRNA in colorectal carcinoma were significantly higher than that in the normal tissues from resection margin(P < 0.01).Conclusion The high expression of MMP-14 and CD31-MVD in colorectal carcinoma were closely related to the occurrence,invasion and metastasis of colorectal cancer.
Keywords:colorectal cancer  MMP-14  CD31-MVD  immunohistochemistry  qRT-PCR
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