ARL4C在睾丸生殖细胞肿瘤中的表达及临床意义

目的:探讨ADP-核糖基化因子-4C（ARL4C）在睾丸生殖细胞肿瘤（TGCT）中的表达及其临床意义。方法:收集GEPIA数据库及天津医科大学第二医院2015-2019年睾丸生殖细胞肿瘤病人临床及病理信息，探索ARL4C在睾丸生殖细胞肿瘤中的表达情况及其对预后的影响，免疫组化、RT-qPCR及Western验证，通过STRING数据库寻找其可能的作用机制。结果:与正常组织相比，ARL4C在胆管癌、多形性胶质母细胞瘤、肾透明细胞癌、急性髓系白血病、肺鳞状细胞癌、卵巢浆液性囊腺癌、胰腺癌、嗜铬细胞瘤和副神经节瘤、胃腺癌、睾丸生殖细胞肿瘤、胸腺瘤、子宫内膜癌等多种恶性肿瘤中均呈现高表达状态（均P＜0.05），在睾丸生殖细胞肿瘤中生存分析显示高表达ARL4C的患者生存时间减少（P＜0.05），GEPIA数据库提示ARL4C与DIRAS1有相互作用，相关系数为0.45（P＜0.05）。免疫组化验证DIRAS1在睾丸生殖细胞肿瘤中呈现低表达状态。结论:ARL4C在睾丸生殖细胞肿瘤中高表达，且其高表达与预后不良相关。

Expression and clinical significance of ARL4C in testicular germ cell tumors

Objective: To investigate the expression and clinical significance of ADP-ribosylation factor-4C (ARL4C) in testicular germ cell tumors (TGCT). Methods: The clinical and pathological information of patients with testicular germ cell tumors in GEPIA database and Tianjin Medical University Second Hospital from 2015 to 2019 were collected to explore the expression of ARL4C in testicular germ cell tumors and its impact on prognosis. The expression was validated by immunohistochemistry, RT-qPCR and Western bolt. The possible mechanism was found by STRING database. Results: Compared with normal tissues, ARL4C showed high expression status in cholangiocarcinoma, glioblastoma multiforme, renal clear cell carcinoma, acute myeloid leukemia, lung squamous cell carcinoma, ovarian serous cystadenocarcinoma, pancreatic cancer, chromaffin cellular and paraganglioma, gastric adenocarcinoma, testicular germ cell tumor, thymoma, endometrial cancer and other malignant tumors(all P＜0.05). Survival analysis in testicular germ cell tumors showed that patients with high expression of ARL4C had decreased survival time (P＜0.05). The GEPIA database suggested that ARL4C interacted with DIRAS1 and correlation coefficient was 0.45(P＜0.05). Immunohistochemistry confirmed that DIRAS1 showed a low expression status in testicular germ cell tumors. Conclusion: ARL4C is highly expressed in testicular germ cell tumors, and its high expression is associated with poor prognosis.