银屑病患者皮损间充质干细胞的培养鉴定及其表达HES1和CXCL6的研究

【摘要】 目的 培养鉴定银屑病患者皮损处真皮间充质干细胞（DMSC），并研究DMSC的发状分裂相关增强子1（HES1）和趋化因子配体6（CXCL6）表达情况。方法 分离培养15例银屑病患者皮损及18例正常人皮肤的DMSC，利用流式细胞仪进行表型鉴定，实时荧光定量聚合酶链反应（RT-PCR）及免疫印迹（Western blot）检测HES1和CXCL6的mRNA及蛋白表达水平，两组间比较采用t检验。结果 银屑病组DMSC形态与正常对照组无差异，但HES1 和CXCL6基因的mRNA水平分别是对照组的3.56和3.44倍，且差异有统计学意义（P < 0.05）。在蛋白水平，银屑病组DMSC中HES1和CXCL6的表达明显高于对照组（P < 0.05）。结论 银屑病患者皮肤DMSC HES1及CXCL6表达升高可能参与银屑病的发病。

Culture and identification of dermal mesenchymal stem cells from skin lesions of patients with psoriasis and analysis of HES1 and CXCL6 expression in these cells

【Abstract】 Objective To culture and identify dermal mesenchymal stem cells （DMSCs） in skin lesions of patients with psoriasis, and to determine the expression of hairy and enhancer of split-1（HES1）and chemokine ligand 6 （CXCL6） in DMSCs. Methods DMSCs were isolated from skin lesions of 15 patients with psoriasis and normal skin tissues of 18 healthy controls, and then subjected to culture. Cell phenotypes were identified by flow cytometry, and mRNA and protein expression of HES1 and CXCL6 was determined by real-time fluorescence-based quantitative PCR （RT-PCR） and Western blot analysis respectively. Comparisons were performed between 2 groups by using t test. Results There was no difference in the morphology of DMSCs between the psoriasis group and control group. The mRNA expression of HES1 and CXCL6 in the psoriasis group was 3.56 and 3.44 times that in the control group respectively, and there was a significant difference between the two groups （both P < 0.05）. The protein expression of HES1 and CXCL6 in DMSCs was significantly higher in the psoriasis group than in the control group （both P < 0.05）. Conclusion The high expression of HES1 and CXCL6 in DMSCs from lesions may be involved in the occurrence of psoriasis.