miR-212 在宫颈癌中的表达及对癌细胞增殖和侵袭的影响与机制

目的 探讨miR-212在宫颈癌中的表达及其对宫颈癌细胞增殖和侵袭的影响及作用机制。方法 采用RT-PCR和Western Blot方法检测宫颈癌细胞中miR-212和TCF7L2的表达水平。应用BrdU细胞增殖检测和Transwell侵袭实验分别检测细胞增殖和侵袭情况。运用生物信息学方法Target Scan预测并筛选miR-212的蛋白编码基因靶点,并应用双荧光素酶报告系统进行验证。结果 与癌旁组织相比,miR-212在宫颈癌组织中的表达下调(P<0.01);与End1/E6E7细胞相比,miR-212在宫颈癌细胞系中的表达水平均显著降低(P<0.01)。miR-212的过表达可以抑制细胞的增殖和侵袭,而低表达miR-212可以促进宫颈癌细胞系的增殖和侵袭(P<0.01)。miR-212的过表达可以显著抑制TCF7L2蛋白的表达,而miR-212的低表达则促进TCF7L2蛋白的表达。靶基因TCF7L2是miR-212的直接作用靶点。结论 宫颈癌组织中miR-212表达降低,通过基因TCF7L2的靶向调节抑制宫颈癌的侵袭和转移。

Expression of microRNA-212 in cervical cancer and its effect on proliferation and invasion of cancer cells

Objective To investigate the expression of miR-212 in cervical cancer and its effect on proliferation and invasion of cervical cancer cells and its mechanism. Methods RT-PCR and Western Blot were used To detect the expression levels of miR-212 and TCF7L2 in cervical cancer cells. BrdU cell proliferation assay and Transwell invasion assay were used to detect cell proliferation and invasion. Target Scan was used to predict the protein-coding gene target of miR-212, and double luciferase reporting system was used to validate it. Results The expression of mir-212 in cervical cancer tissues was down-regulated, compared with that in adjacent tissues (P<0.01). Compared with End1/E6E7 cells, the expression level of mir-212 in cervical cancer cell lines was significantly reduced (P<0.01). Overexpression of miR-212 can inhibit cell proliferation and invasion, while low expression of miR-212 can promote the proliferation and invasion of cervical cancer cell lines (P<0.01). Overexpression of miR-212 can significantly inhibit the expression of TCF7L2 protein, while low expression of miR-212 can promote the expression of TCF7L2 protein. The target gene TCF7L2 is the direct target of miR-212. Conclusion The expression of miR-212 in cervical cancer tissue was decreased, and the invasion and metastasis of cervical cancer were inhibited by targeting regulation of TCF7L2 gene.