基于网络药理学的黄芪抗心力衰竭作用机制

目的 基于网络药理学研究黄芪抗心力衰竭的作用机制。方法 采用活性成分筛选与靶点预测技术,结合生物信息学手段,预测黄芪抗心力衰竭的潜在作用靶点,并进行信号通路分析,从而探讨其治疗心力衰竭的分子机制。结果 在TCMSP数据中搜索筛选得到20个相应黄芪化学成分,同时利用靶点预测技术共筛选出相关靶点121个。同时在疾病基因数据库检索得到10 962个与心力衰竭发生、发展有关的已知靶点基因,利用string数据库和Cytoscape软件中的网络拓扑分析共筛选获取关键靶点8个。GO富集分析显示,黄芪可通过参与细胞能量代谢、血管内环境变化、神经活性配受体作用、激素活性等10个生物功能的PI3K/AKT、p38丝裂原活化蛋白激酶(p38MAPK)和MAPK/NF-κB等10条关键信号通路发挥抗心力衰竭的作用。结论 中药黄芪抗心力衰竭的作用具有多成分、多靶点、协同作用的特点。

Study on treatment mechanism of heart failure for astragali radix based on network pharmacology

Objective To study the mechanism of astragali radix against heart failure based on network pharmacology.Methods The potential target of astragali radix against heart failure and signal pathways were predicted by active ingredient screening and target prediction technology, combined with bioinformatics. Finally, we explored the molecular mechanism of astragali radix in the treatment of heart failure.Results Twenty corresponding chemical constituents in astragali radix were screened through the TCMSP data, and 121 targets of these components were found using target prediction technology. Meanwhile, 10 962 known genes related to the occurrence and development of heart failure were retrieved from the gene databases. Eight key targets were selected by string database screening and network topology analysis in Cytoscape software. GO enrichment analysis showed that astragali radix played an important role in anti-heart failure by participating in 10 key signal pathways such as PI3K/AKT, p38MAPK, and MAPK/NF-κB, which were related to 10 biological functions including Cellular energy metabolism response, changes in blood vessel environment, hormone activity, nerve activity and receptor function.Conclusion As a traditional Chinese medicine, the effect of astragali radix against heart failure has the characteristics of multi-component, multi-target and mutual synergy.