lncRNA TTN-AS1靶向抑制miR-1271的表达对肺癌细胞增殖、侵袭和凋亡的影响及机制

目的：探讨lncRNA TTN-AS1调控miR-1271表达对肺癌细胞增殖、侵袭和凋亡的影响及机制。方法：在人肺癌A549细胞中转染TTN-AS1 siRNA或miR-1271 mimics，采用qRT-PCR检测TTN-AS1和miR-1271的表达，CCK-8实验检测细胞增殖，Transwell实验检测细胞侵袭，流式细胞术检测细胞凋亡，Western blot检测PI3K、p-AKT、PCNA、E-cadherin和cleaved caspase 3表达。共转染miR-1271 inhibitors和TTN-AS1siRNA，观察抑制miR-1271对抑制TTN-AS1诱导的A549细胞增殖、侵袭和凋亡的影响。双荧光素酶报告基因实验检测TTN-AS1和miR-1271及miR-1271和PDK1的靶向作用关系，Western blot分析过表达或抑制TTN-AS1/miR-1271对PDK1表达的影响。结果：抑制TTN-AS1及过表达miR-1271均可明显抑制A549细胞增殖和侵袭能力，促进细胞凋亡，下调PI3K、p-AKT和PCNA表达，上调E-cadherin和cleaved caspase 3表达（P ＜0.05）。抑制miR-1271可逆转抑制TTN-AS1对A549细胞增殖和侵袭能力的抑制作用以及对细胞凋亡的促进作用。TTNAS1和PDK1与miR-1271均存在靶向调控关系。结论：lncRNA TTN-AS1可通过靶向调节miR-1271/PDK1并抑制PI3K/AKT信号通路降低A549细胞增殖和侵袭能力，促进细胞凋亡。

The effect of lncRNA TTN-AS1 targeted inhibition of miR-1271 expression on the proliferation,invasion and apoptosis of lung cancer cells and its mechanism

Objective: To investigate the effect of lncRNA TTN-AS1 on the proliferation, invasion and apoptosis of lung cancer cells by regulating miR-1271 expression. Methods: Human lung cancer A549 cells were transfected with TTN-AS1 siRNA or miR-1271 mimics. QRT-PCR was used to detect the expression of TTNAS1 and miR-1271, CCK-8 test to detect cell proliferation, Transwell test to detect cell invasion, flow cytometry to detect cell apoptosis and Western blot to detect the expression of PI3K, p-AKT, PCNA, E-cadherin and cleaved caspase 3. miR-1271 inhibitors and TTN-AS1 siRNA were co-transfected and the effect of miR-1271 inhibition on the proliferation, invasion and apoptosis of A549 cells induced by TTN-AS1 was observed. The dual luciferase reporter gene experiment was performed to detect the targeting effect of TTN-AS1/miR-1271 and miR-1271/PDK1. The effect of over-expression or inhibition of TTN-AS1/miR-1271 on PDK1 expression was analyzed by Western blot. Results: Inhibition of TTN-AS1 and over-expression of miR-1271 could significantly inhibit the proliferation and invasion of A549 cells, promote apoptosis, down-regulate the expression of PI3K, p-AKT and PCNA, and up-regulate the expression of E-cadherin and cleaved caspase 3 (P<0.05). Inhibition of miR-1271 could reverse the inhibitory effect of TTN-AS1 on the proliferation, invasion of A549 cells and the promotion of apoptosis as well. Both TTN-AS1 and PDK1 had a targeted regulatory relationship with miR-1271. Conclusion:lncRNA TTN-AS1 can inhibit the proliferation and invasion of A549 cells and promote apoptosis by targeting miR-1271/PDK1 and inhibiting PI3K/AKT signaling pathway.