| PCR检测血清中HBV—DNA及其临床意义 |
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| 引用本文： | 郑祥雄 胡金福. PCR检测血清中HBV—DNA及其临床意义[J]. 中国人兽共患病杂志, 1993, 9(1): 20-21 |
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| 作者姓名： | 郑祥雄 胡金福 |
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| 作者单位： | 福建医学院附属协和医院(郑祥雄，黄淑玉，陈道纯，蔡跃生)，福建省卫生防疫站(胡金福)，福建省卫生防疫站(张立农) |
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| 摘 要： | 应用聚合酶链反应(PCR)结合Southern blot核酸杂交和DNA直接杂交技术,检测50例乙型肝炎病毒(HBV)感染后不同血清学标志的血清标本及5例无任何HBV血清学标志的血清标本中的HBV DNA,并与血清学检查结果比较。结果发现PCR结合Southern blot核酸杂交的灵敏度明显高于DNA直接杂交技术,而且还能直接反映患者血液是否有感染性。此外,前者还能直接反映病毒在体内的复制情况,澄清模棱两可的血清学结果。
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| 关 键 词： | 聚合酶链反应 DNA 乙型肝炎病毒 |
Detection of Hepatitis B Virus in Serum by Polymerase Chain Reaction |
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| Zheng Xiangxiong et al. Detection of Hepatitis B Virus in Serum by Polymerase Chain Reaction[J]. Chinese Journal of Zoonoses, 1993, 9(1): 20-21 |
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| Authors: | Zheng Xiangxiong et al |
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| Affiliation: | Zheng Xiangxiong et al |
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| Abstract: | 50 cases of sera with different patterns of Hepatitis B virus (HBV) infection and 5 witho any HBV marker were tested for HBV DNA both by PCR-Southern blotting and by direct hy bridization. The results showed that the sensitivity of PCR-Southern blotting was obvious higher than that of direct hybridization. It, was possible not only to demonstrate infectivity of person' s serum,but also to rule it out .In addition , PCR-Southern blotting was helpful in r vealing the state of HBV replication and in clarifying ambiguous serological results. |
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| Keywords: | Polymerase chain reaction HBV DNA DNA direct hybridization. |
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