顶空固相微萃取-气质联用分析酸枣仁挥发性成分

目的:建立一种对酸枣仁中挥发性成分快速分析的简单方法。方法:采用顶空固相微萃取法萃取酸枣仁挥发性成分,优化萃取条件,采用GC-MS联用技术进行成分分析。结果:样品在90℃下预热30 min,选用65μmPDMS/DVB(聚二甲氧基硅烷/二乙烯基苯)涂层纤维头,顶空萃取50 min,250℃下脱附5 min,在中等极性柱RxiTM-50上分离,共分离出126种化合物,定性116种;采用峰面积归一化法计算各组分含量:其主要成分为烷烃类(32.08%)、萜类及其含氧衍生物(27.06%)。结论:该方法萃取物质种类多,检索匹配度高,重复性好,适合于酸枣仁挥发性成分的快速测定。

Analysis on volatile constituents of Semen Ziziphi Spinosae by HS-SPME-GC-MS

Objective:To establish a rapid and simple method for the determination of volatile constituents in Semen Ziziphi Spinosae.Methods:The volatile constituents in Semen Ziziphi Spinosae were extracted by Head Space Solid-Phase Microextraction(HS-SPME) and analysed by GC-MS.Results:Powder of sample was pre-heated for 30 min at 90 ℃,then headspace-extracted with 65 μm PDMS/DVB fiber for 50 min.After desorbed for 5 min at 250 ℃,the separation was well completed on a RxiTM-50 capillary column.126 kinds of volatile compounds were isolated and 116 compounds were identified.The amounts of compound from the volatile constituents were determined by area normalization method.The main components extracted by HS-SPME were alkane(32.08%),terpenoid and derivatives of oxygenated terpenoid(27.06%).Conclusion:The method is simple,fast and accurate,and it is suitable for the determination of the volatile constituents in fruit of Ziziphus jujuba.