| 缺氧和机械损伤诱导大鼠神经细胞凋亡的体外研究 |
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| 引用本文： | 姜曙,于如同,鞠延,贺民,毛伯镛,高立达. 缺氧和机械损伤诱导大鼠神经细胞凋亡的体外研究[J]. 中华创伤杂志, 2000, 16(11): 669-671 |
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| 作者姓名： | 姜曙 于如同 鞠延 贺民 毛伯镛 高立达 |
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| 作者单位： | 1. 华西医科大学附属第一医院神经外科,成都,610041
2. 徐州医学院附属第一医院神经外科 |
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| 摘 要： | 目的 探讨神经细胞调亡与缺氧和机械损伤的关系。方法 体外原代培养大鼠神经元细胞,经低氧或机械损伤处理后,应用光镜、电镜、末端脱氧核苷酸介导的Ｘ－ｄＵＴＰ缺口末端标记（ＴｄＴ－ｍｅｄｉａｔｅｄ ｄＵＴＰ ｎｅｉｋ ｅｎｄ ｌａｂｅｌｉｎｇ,ＴＵＮＥＬ）法、流式细胞仪、ＤＮＡ凝胶电泳及免疫组化分析细胞凋亡及其相关基因表达的改变。结果 经低氧或机械损伤后神经细胞出现恨的形态改变;其ＤＮＡ呈现“梯状”断裂
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| 关 键 词： | 缺氧 创伤 基因表达 机械损伤 神经细胞调亡 |
| 修稿时间： | 1999-11-08 |
In vitro study on neuronal apoptosis induced by hypoxia or traumatic injury in rats |
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| JIANG Shu ,YU Rutong,JU Yan,et al.. In vitro study on neuronal apoptosis induced by hypoxia or traumatic injury in rats[J]. Chinese Journal of Traumatology, 2000, 16(11): 669-671 |
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| Authors: | JIANG Shu YU Rutong JU Yan et al. |
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| Affiliation: | JIANG Shu *,YU Rutong,JU Yan,et al. * Dept. of Neurosurgery,First Affiliated Hospital to West China University of Medical Sciences,Chengdu 610041,China |
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| Abstract: | Objective To explore the relationship between hypoxia or traumatic injury and neuronal apoptosis. Methods Rat neurons primarily cultured in vitro were made to be with hypoxia or traumatic injury. The neuronal apoptosis was evaluated with microscope, TUNEL staining, flow cytometry, agarose gel electrophoresis and immunohistochemistry. Results Morphological changes of apoptosis appeared in the treated neurons. DNA fragmentation showed a ladder break. The apoptosis ratio was 10.8% in hypoxia group and 4.8% in trauma group, while it was only 1.6% in the control group. The expression of apoptotic associate genes (c-myc, fas and fasL) was increased. Conclusions Hypoxia or traumatic injury can induce neuronal apoptosis; and its molecular mechanism is possibly related to expression of apoptotic associate genes. |
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| Keywords: | Apoptosis Hypoxia Wounds and injuries Gene expression |
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