家蝇幼虫抗弓形虫抗菌肽分离纯化

目的 从家蝇幼虫血淋巴中分离纯化具有抗弓形虫作用的抗菌肽.方法 通过损伤加感染的方法诱导家蝇幼虫大量表达抗菌肽,然后经过研磨、离心和层析等过程,将家蝇幼虫抗菌肽进行分离纯化,采用四甲基偶氮噻唑蓝(MTT)比色法和血细胞计数法筛选对弓形虫速殖子有抑制作用的抗菌肽.结果 经Resource S阳离子柱和Su-perdex G75凝胶柱层析后,筛选出2种对弓形虫速殖子有杀伤作用的抗菌肽.结论 家蝇幼虫血淋巴中存在抗弓形虫作用的抗菌肽,而且不止一种,但其效果有所不同.

Purification of the antimicrobial peptides with anti-toxoplasma gondii effect isolated from Musca domestica larvae

Objective To isolate and purify the antimicrobial peptides with anti-toxoplasm a gondii activity isolated from the haemlymph of M usca domestica larvae.Methods The antimicrobial peptides of Musca domestica larvae were induced by infection and injury were isolated and purified by trituration,centrifugalization and column chromatography.Then the antimicrobial peptides with anti-toxoplasma activity were sieved by M TT colorimetric method and haemacytometry.Results It was found that two antimicrobial peptides had anti-toxoplasma activity through Resource Scationic column chromatography and Superdex G75 gel column chromatography.Conclusion The two antimicrobial peptides with anti-toxoplasma activity existing in the haemlymph of M usca domestica larvae had different anti-toxoplasma effect.