甲状旁腺激素促进系膜细胞合成分泌转化生长因子β1

目的：研究甲状旁腺激素（PTH）对大鼠系膜细胞合成与分泌转化生长因子β1（TGF－β1）的影响。方法：（1）分别以10^-12,10^-11,10^-10,10^-9,10^-8mol/l的hPTH1-34刺激大鼠系膜细胞6，12，24，48h后，ELISA方法测定上清中TGF－β1的浓度；（2）分别以10^-12,10^-11,10^-10,10^-9,10^-8mol/L的hPTH1-34刺激大鼠系细胞48h,采用半定量RT－PCR方法检测细胞TGF－β1 mRNA的表达；（3）以10^-8mol/L的hPTH1-34分别刺激大鼠系膜细胞6，12，24，48h，采用半定量RT－PCR方法检测细胞TGF－β1 mRNA的表达，结果：（1）ELISA结果显示，hPTH1-34促进大鼠系膜细胞合成与分泌TGF－β1分泌作用达高峰（P＜0．01），（2）半定量RT－PCR方法结果显示，hPTH1-34促进大鼠系膜细胞TGF－β1 mRNA的表达，并具有浓度依赖和时间依赖性特点（各组与对照组间P＜0．05），结论：hPTH1-34从蛋白和基因水平显著促进系膜细胞TGF－β1的合成与分泌，且呈浓度依赖和时间依赖性特点。

Parathyroid hormone regulates the synthesis of transforming growth factor beta 1 in cultured mesangial cells of rats

Objective To identify whether PTH can regulates the synthesis and secretion of TGF-B1 in cultured mesangial cells of rats. Methods (1) Mesangial cells seeded at a density of 1 X 104 cells/well in 24-well plates were treated with medium containing various concentrations of hPTH1-34 (10-12 - 10-8mol/L) for different time (6 h, 12 h, 24 h and 48 h) ; while control cells were treated with vehicle only. Then TGF-B1 levels of the supernatants were measured by ELISA. (2) Mesangial cells seeded at a density of 3 x 105 cells/well in 6-well plates were treated with medium containing various concentrations of hPTH1-34(10-12- 10-8mol/L) .48 hours later, TGF-BlmRNA was tested by half quantitative RT-PCR. (3) The same way as before, cells were treated with 10-8mol/L hPTH1-34 for 6, 12, 24, 48 hours. Then TGF-BlmRNA was tested by half quantitative RT-PCR. Results (1)hPTH1-34 stimulated TGF-B1 synthesis in a dose- and time-dependent way with a peak at 10-8mol/L( P < 0. 01) . (2) hPTH1-34 increased the expression of TGF-B1 mRNA of cultured rat mesangial cells in a dose-and time-dependent manner ( P < 0.05) . Conclusion hPTH1-34 up-regulates the protein synthesis and mRNA expression of TGF-B1 in cultured mesangial cells of rats.