| 薏苡仁酯对胃癌BGC-823细胞侵袭迁移能力的影响及其机制 |
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| 引用本文： | 王敏,姜藻. 薏苡仁酯对胃癌BGC-823细胞侵袭迁移能力的影响及其机制[J]. 东南大学学报(医学版), 2010, 29(3): 283-286. DOI: 10.3969/j.issn.1671-6264.2010.03.011 |
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| 作者姓名： | 王敏 姜藻 |
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| 作者单位： | 东南大学附属中大医院,临床肿瘤中心,江苏,南京,210009 |
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| 基金项目： | 江苏省卫生厅医学科技发展基金(P200914) |
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| 摘 要： | 目的:研究不同浓度薏苡仁酯对胃癌BGC-823细胞株的侵袭、迁移能力及CD44、CD133表达的影响。方法:以不同浓度薏苡仁酯作用于BGC-823细胞,MTT法检测细胞增殖抑制率,黏附试验、Transwell小室侵袭迁移实验检测细胞黏附、侵袭、迁移能力,RT-PCR法检测细胞CD44、CD133 mRNA表达水平。结果:薏苡仁酯可抑制BGC-823细胞增殖,其抑制效应呈剂量-时间依赖性;经薏苡仁酯作用后BGC-823细胞的侵袭、迁移及黏附能力降低;CD44、CD133 mRNA的表达与薏苡仁酯浓度亦呈负相关。结论:薏苡仁酯能降低胃癌BGC-823细胞株的侵袭、迁移、黏附能力,其机制可能与通过下调CD44、CD133表达有关。
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| 关 键 词： | 薏苡仁酯 胃癌 侵袭 迁移 黏附 CD44 CD133 |
Effects of coixenolide on invasion and metastasis capacity of gastric carcinoma BGC-823 cells in vitro and its mechanism |
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| WANG Min,JIANG Zao. Effects of coixenolide on invasion and metastasis capacity of gastric carcinoma BGC-823 cells in vitro and its mechanism[J]. Journal of Southeast Univ: Medical Sci Ed, 2010, 29(3): 283-286. DOI: 10.3969/j.issn.1671-6264.2010.03.011 |
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| Authors: | WANG Min JIANG Zao |
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| Affiliation: | Department of Oncology/a>;Zhongda Hospital/a>;Southeast University/a>;Nanjing 210009/a>;China |
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| Abstract: | Objective:To investigate the effect of coixenolide on adhesion,invasion and metastasis of gastric carcinoma BGC-823 cells in vitro and its mechanism.Methods:MTT assay was used to examine the effect of coixenolide on proliferation of BGC-823 cells.Matrigel adhesion assay and Transwell chamber were performed to determine the adhesion,invasion and migratory capacity of the cells.RT-PCR was applied to detect the expression of CD44 and CD133 mRNA in BGC-823 cells.Results:Coixenolide could inhibit the proliferati... |
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| Keywords: | coixenolide gastric cancer invasion metastasis adhesion CD44 CD133 |
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