白藜芦醇对食管癌细胞凋亡相关基因survivin和bax表达的影响

[目的]探讨白藜芦醇诱导食管癌Eca109细胞凋亡的作用机制。[方法]MTT比色法测定细胞增殖的抑制率；透射电镜下观察细胞凋亡形态学变化；RT-PCR法和流式细胞术（FCM）检测白藜芦醇对Eca109细胞survivin和bax表达的影响。[结果]白藜芦醇对Eca109细胞生长有抑制作用．抑制率达76．42％．并呈剂量一时间效应关系。白藜芦醇处理细胞后可见凋亡形态学变化，部分细胞体积变小。染色质浓缩及边集。RT—PCR法和FCM检测结果显示白藜芦醇可抑制survivin上调，促进bax的表达。[结论]白藜芦醇能诱导食管癌Eca109细胞凋亡，其机制可能与调节survivin和bax的表达有关。

Impact of Resveratrol on the Expression of Apoptosis Related Gene Survivin and Bax in Human Esophageal Cancer Cells

[Purpose] To explore the mechanism of apoptosis in human esophageal cancer Eca109 ceils by resveratrol. [Methods] The suppressive ratio of resveratrel on Eca109 cells proliferation was evaluated by MTT colorimetric assay and morphology was observed by transmission electron microscope. The expression of survivin and bax was analyzed by RT-PCR and Flow Cytometry （FCM）. [Results] Resveratro] inhibited the growth of Eca109 ceils in a dose-and time-dependent manner, and the suppressive ratio arrived at 76.42%. Morphological apoptosis could be observed after treated with resveratrol. The bulk of some drug-treated cells turned small and the nuclear chromatin became condensed and marginated. The results determined by RT-PCR and FCM showed that resveratrol could down-regulate survivin, while upregulate bax. [Conclusion] Resveratrol could induce the apoptosis of human esophageal cancer Eca109 cells, and its possible molecular mechanisms might be related to modulation the expression of survivin and bax.