玉米肽对微波辐射大鼠肝组织损伤的治疗作用

目的：探讨玉米肽对微波辐射引起动物肝细胞肝组织损伤的治疗作用。方法36只Wistar大鼠随机分为正常对照组、辐射模型组和辐射给药组，辐射模型组和辐射给药组大鼠给予100 mW/cm2的微波辐照6 min，辐射给药组辐射后给予600 mg/（ kg· d）玉米肽，连续7 d。7 d后处死大鼠，光学显微镜观察肝细胞形态学变化，检测大鼠血清中乳酸脱氢酶（LDH）、丙二醛（MDA）、超氧化物歧化酶（SOD）、谷丙转氨酶（ALT）活性。结果与对照组比较，辐射模型组肝细胞体积增大，细胞核形态不规则，细胞水肿严重，细胞之间界限不明显，胞质淡染，肝血窦明显缩小或消失。血清LDH显著升高（ P＜0．05）， MDA和ALT含量升高极为显著（ P＜0．01），SOD含量极显著下降（P＜0．01）。辐射给药组血清ALT含量显著升高（P＜0．05）。与辐射模型组比较，辐射给药组肝组织结构有明显恢复，肝细胞水肿明显减轻，细胞边界较清晰。血清ALT含量下降极为显著（P＜0．01），SOD活性显著升高（P＜0．05），LDH和MDA呈下降趋势。结论100 mW/cm2的微波辐照可引起大鼠肝组织损伤，玉米肽对微波辐射引起的肝组织损伤有一定的治疗效果。

Treatment of corn peptide on damage of hepatic tissue in microwave radia-tion rats

Objective To probe the treatment effect of corn peptide on hepatic tissues damage in microwave radiation rats.Methods 36 Wistar rats were randomly divided into normal control group , model group and radiation dosage group.100 mW/cm2 microwave radiation were given to model group and radiation dosage group for 6mins.100 mg/（ kg· d） corn peptide were administered to the rats of radiation dosage group for 7 consecutive days .Then the rats were sacrificed.Liver cell morphological changes was observed under light microscopes and the content of MDA ,the activity of LDH,SOD and ALT were determined .Results Compared with the normal control group ,the model group shown that the liver mass were increased ,nuclear shapes was irregular and cell edema was serious ,boundaries between cells was not obvious and cytoplasmic dye was light ,liver blood sinus shrank obviously or disappeared .Serum LDH in-creased significantly （ P〈0 .05 ） , the content of MDA and ALT increased significantly （ P〈0 .01 ） and content of SOD decreased significantly （P〈0.01）.Content of serum ALT of radiation dosage group increased significantly com-pared with the normal control group （P〈0.05）.Compared with the model group,liver tissue structure in the radiation dosage group recovery was obvious ,liver cell edema reduced significantly and cell boundary was clear;the content of serum ALT decreased significantly （ P〈0 .01 ） , and activity of SOD increased significantly （ P 〈0 .05 ） , LDH and MDA were in decline .Conclusion Microwave irradiation of 100 mW/cm2 can cause liver tissue injury in rats and corn peptide has a certain therapeutic effect on liver tissue damage caused by microwave radiation .effect on liver tissue damage caused by microwave radiation .