超高效液相色谱法同时测定维生素强化食品中多种水溶性维生素

目的建立可同时测定维生素强化食品中7种水溶性维生素的超高效液相色谱法。方法采用梯度洗脱超高效液相色谱法，Waters ACQUITY UPLC^TM BEH C18柱（2．1mm×50mm，1．7μm），流动相A为辛烷磺酸钠0．8g／L水溶液（冰乙酸调pH=2．8）；B为甲醇，检测波长270nm，流速0．4ml／min。结果各种维生素含量测定方法的线性关系良好，r=0．9992—0．9998，回收率98．1％～100．2％，RSD=0．17—0．72。用该法测定了市售维生素饮料和维生素强化食品中维生素的含量，结果准确。结论本方法操作简便，分析速度快，灵敏度高，精密度好，结果准确可靠。

The Simultaneous Determination of Water-soluble Vitamins in Fortified Feeds by Ultra Performance Liquid Chromatography

Objective To establish ultra performance liquid chromatography（UPLC） method for the analysis of vitamin B1 ,B2, B6, C,folic acid, nicotinic acid and nicotinamide. Methods UPLC was adapted on Waters ACQUITY UPLC^TM BEH C18 column（2. 1 mm ×50 mm,1.7 μm） ,the mobile phase consisted of A：0. 8 g/L 1 - Octanesulfonic acid sodium salt hydrate and B： methanol, the detection was set at 270 nm, and the flow rate was 0. 4 ml/min. Results In the linear range the correlation coefficients ranged from 0. 999 3 to 0. 999 8. The average recoverie rates were 98. 1% - 100. 2% with corresponding RSD of 0. 17 - 0. 72. The method was successfully applied for determination of vitamin in vitamin beverages and fortified foods. Conclusion This method is proved to be of good speed,specificity,sensitivity and accuracy.