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| 右美托咪定对脂多糖诱导A549细胞表面活性蛋白的影响 | |
| 引用本文： | 朱军,彭晓红. 右美托咪定对脂多糖诱导A549细胞表面活性蛋白的影响[J]. 武汉大学学报(医学版), 2015, 36(1): 36-38 |
| 作者姓名： | 朱军 彭晓红 |
| 作者单位： | 武汉市普爱医院麻醉科 |
| 摘 要： | 目的:观察不同浓度右美托咪定对A549细胞表达表面活性蛋白的影响。方法:体外培养的A549细胞经脂多糖诱导制备急性肺损伤细胞模型。分为对照组和实验组(LPS,0.01,0.10,1.00μmol/L),作用6h。四甲基偶氮唑盐法观察各组细胞增殖情况,酶联免疫吸附法检测各组细胞裂解液中表面活性蛋白的含量。结果:右美托咪定对A549细胞的存活率无明显影响(P>0.05);随右美托咪定浓度增大,A549细胞表达表面活性蛋白也逐渐增多(P<0.05)。结论:右美托咪定能促进A549细胞表面活性蛋白的表达,对A549细胞具有一定的保护作用。 |
| 关 键 词： | 急性肺损伤 右美托咪定 肺表面活性蛋白 |
Effect of Dexmedetomidine on Surfactant Protein of Lipopolysaccharide Induced A549 Cells |
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| ZHU Jun;PENG Xiaohong. Effect of Dexmedetomidine on Surfactant Protein of Lipopolysaccharide Induced A549 Cells[J]. Medical Journal of Wuhan University, 2015, 36(1): 36-38 | |
| Authors: | ZHU Jun PENG Xiaohong |
| Affiliation: | ZHU Jun;PENG Xiaohong;Dept.of Anesthesiology,Pu’ai Hospital of Tongji University; |
| Abstract: | Objective:To evaluate the effect of dexmedetomidine on surfactant protein of A549 cells with acute lung injury(ALI).Methods:A549cells were exposed to lipopolysaccharide to make the cell model of ALI which were cultured invitro.The ALI-A549 cells were exposed to 1.0×106/ml ALI-A549 cell suspension and 0.01,0.10,1.00μmol/L dexmedetomidine for 6hours.We observed the proliferation of the cells by methylthiazolyltetrazolium method,and content of surfactant protein of the cells in lysate were assayed by enzyme-linked immunosorbent assay in each group.Results:Dexmedetomidine significantly improved the survival rate of ALI-A549cells(P<0.05),this effect was concentration-dependent;Surfactant protein gradually increased with concentration of the dexmedetomidine increasing(P<0.05).Conclusion:Dexmedetomidine has a protective effect for ALI-A549 cells by promoting the expression of surfactant proteins. |
| Keywords: | Acute Lung Injury Dexmedetomidine Pulmonary Surfactant Protein |
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