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| 自体骨髓间充质干细胞促进大鼠坐骨神经缺损修复的实验研究 | |
| 引用本文： | 廖文,刘淑红,张玉富,刘慧哲,李章华,赵永歧,范文红,夏仁云,罗永湘,范明. 自体骨髓间充质干细胞促进大鼠坐骨神经缺损修复的实验研究[J]. 华中科技大学学报(医学版), 2004, 33(4): 459-463 |
| 作者姓名： | 廖文 刘淑红 张玉富 刘慧哲 李章华 赵永歧 范文红 夏仁云 罗永湘 范明 |
| 作者单位： | 1. 军事医学科学院基础医学研究所,北京,100850;华中科技大学同济医学院附属同济医院骨科,武汉,430030 2. 军事医学科学院基础医学研究所,北京,100850 3. 华中科技大学同济医学院附属同济医院骨科,武汉,430030 4. 河北职工医学院附属医院肌电图室,保定,071000 |
| 基金项目： | 国家“十五”863生物技术领域课题基金资助项目(No2001AA216031)，北京市二四八工程重大创新基金资助项目(NoH010210190123) |
| 摘 要： | 目的 观察自体骨髓间充质干细胞在神经再生室中的作用 ,探讨其作为组织工程的种子细胞修复周围神经缺损的可行性。方法 成年Wistar大鼠 2 4只 ,随机分成 3组 ,每组 8只 ,硅胶导管桥接右侧 13mm的坐骨神经缺损 ,A组在神经再生室内植入骨髓间充质干细胞 ECM (extracellularmatrix ,ECM )凝胶 ;B组植入ECM凝胶 ;C组注入生理盐水。术后 12周 ,进行大体观察及坐骨神经功能指数测定、电生理检测、神经组织学等观察。结果 A、B两组均有再生神经生成 ,但A组各项检测指标均优于B组 (P <0 0 5或 0 0 1)。结论 自体骨髓间充质干细胞能够促进周围神经再生 ,可以作为种子细胞应用于外周神经组织工程。 |
| 关 键 词： | 骨髓间充质干细胞 大鼠 坐骨神经 神经缺损 神经修复 组织工程 |
| 修稿时间： | 2003-10-29 |
An Experimental Study of Autologous Mesenchymal Stem Cells Promoting Peripheral Nerve Regeneration in Adult Rats |
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| Liao Wen ,,Liu Shuhong,Zhang Yufu et al Institute of Basic Medical Sciences,Academy of Military Medical Sciences,Beijing. An Experimental Study of Autologous Mesenchymal Stem Cells Promoting Peripheral Nerve Regeneration in Adult Rats[J]. Journal of Huazhong University of Science and Technology(Health Sciences), 2004, 33(4): 459-463 | |
| Authors: | Liao Wen Liu Shuhong Zhang Yufu et al Institute of Basic Medical Sciences Academy of Military Medical Sciences Beijing |
| Affiliation: | Liao Wen 1,2,Liu Shuhong1,Zhang Yufu2 et al 1 Institute of Basic Medical Sciences,Academy of Military Medical Sciences,Beijing 100850 2 Department of Orthopaedics,Tongji Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430030 |
| Abstract: | Objective To evaluate the feasibility of using autologous mesenchymal stem cells as seed cells to repair peripheral nerve defects in a adult rat model. Methods Twenty-four adult Wistar rats were divided into 3 groups equally. A 13 mm-long nerve defect was made in the right sciatic nerve and was bridged by a 16 mm-long silicone tube. The silicone tube was filled with the compound of autologous mesenchymal stem cells and extracellular matrix (ECM) gel for group A, ECM gel for group B and saline for group C. Gross observation, electrophysiological examination, hisotological staining and measurement of nerves sciatic nerve function index were carried out at 12 weeks post-operation. Results At 12 weeks post-operation, regenerated nerve existed at both group A and group B. However, each mesurement index in group A was superior to that in group B (P<0.05 or P<0.01). Conclusion Autologous mesenchymal stem cells can promote peripheral nerve regeneration and can be used as seed cells for nerve tissue engineering. |
| Keywords: | nerve regeneration mesenchymal stem cells tissue engineering |
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