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| 热药疗寒的基因表达谱研究 | |
| 引用本文： | 吴斌,杨丽萍, 张天娥,许锦文,王米渠.热药疗寒的基因表达谱研究[J].中国中药杂志,2006,31(11):914-917. |
| 作者姓名： | 吴斌 杨丽萍 张天娥 许锦文 王米渠 |
| 作者单位： | 1. 复旦大学,华山医院,中西医结合研究所,上海,200040 2. 成都中医药大学中医遗传学研究室,四川 成都 610072 3. 新加坡国立癌症中心,新加坡,169610 |
| 基金项目： | 国家高技术研究发展计划(863计划) |
| 摘 要： | 目的:探讨温热药治疗虚寒证的分子机制。方法:选择1对虚寒证兄妹,用温热药(麻辛附子汤合金匮肾气丸加减)治疗45 d,采有显效妹妹的外周血进行基因芯片实验,应用倍数分析方法进行数据处理,并对结果进行基因功能分类、通路分析等信息挖掘。结果:初步获得276条差异表达基因,与新陈代谢基因密切相关,涉及18条通路。结论:温热药主要影响了代谢基因的表达,疗效与证候的基因表达谱存在明显差异,温热药可能是通过基因网络发挥治疗作用的。 |
| 关 键 词： | 基因芯片 药性 虚寒证 |
| 文章编号： | 1001-5302（2006）11-0914-04 |
| 收稿时间： | 2005-07-14 |
| 修稿时间： | 2005-07-14 |
Research on gene expression of warm-hot drug treating cold syndrome |
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| WU Bin,;YANG Li-ping,;ZHANG Tian-e,;XU Jin-wen,;WANG Mi-qu.Research on gene expression of warm-hot drug treating cold syndrome[J].China Journal of Chinese Materia Medica,2006,31(11):914-917. | |
| Authors: | WU Bin ;YANG Li-ping ;ZHANG Tian-e ;XU Jin-wen ;WANG Mi-qu |
| Institution: | 1. Institute of Integrative Chinese and Western Medicine, Huashan Hospital, Fudan University, Shanghai 200040 China; 2. Laboratory of TCM C, enetics, Chengdu University of Traditional Chinese Medicine, Chengdu 610072 China; 3. National Cancer Centre of Singapore, Singapore 169610 |
| Abstract: | Objective: To study the therapeutic molecular mechanism of the warm-hot drugs treating cold syndrome.Method: A brother and his sister with deficiency-cold syndrome were chosen and treated with appropriate Chinese formula consisted chiefly of warm-hot drugs for 45 days.Then microarray technique was applied for comparing the gene expression difference of sister who had significant effect,the data was dialed with multiple analysis method and the results were mined though gene function and pathway.Result: 276 differential genes were obtained,which were related to metabolism and 18 pathways.Conclusion: Warm-hot drugs work on the gene expression of metabolism.It may be exerting the curative action by gene network and there is distinct difference between gene expression of curative effect and syndrome. |
| Keywords: | microarray drug character deficiency-cold syndrome |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |