| 抗HCV阳性慢性肝病患者HBV感染及前C／C区基因变异 |
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| 引用本文： | 邱望龙 李福元. 抗HCV阳性慢性肝病患者HBV感染及前C／C区基因变异[J]. 中华肝脏病杂志, 1996, 4(4): 220-222 |
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| 作者姓名： | 邱望龙 李福元 |
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| 作者单位： | 广州铁路（集团）公司中心医院，第一军医大学南方医院 |
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| 摘 要： | 探讨ＨＢＶ基因变异在ＨＢｓＡｇ阴性抗ＨＣＶ阳性慢性肝病中的意义。方法用巢式聚合酶链反应（ＰＣＲ）与限制片段长度多态性相结合，对５６例慢性肝病患者６例ＨＢｓＡｇ阴性抗ＨＣＶ阳性（Ａ组）．１９例ＨＢｓＡｇ阳性抗ＨＣＶ阳性（Ｂ组）及３１例单独ＨＢＶ成染（Ｃ组）进行前Ｃ区密码２８终止变异（Ａ８３）和Ｃ区密码９７异亮氨酸亮氨酸变异（Ｌ９７）分析。结果Ａ组和Ｂ组Ａ８３和Ｌ９７变异检出率分别为３３％和４２％显著低于Ｃ组８１％（Ｐ值＜０．００１）：而Ａ组和Ｂ组间无统计学差异（Ｐ值＞０．０５）。结论ＨＢＶ和ＨＣＶ双重感染ＨＢｓＡｇ阴性与Ａ８３和Ｌ９７变异无相关。
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| 关 键 词： | 乙型肝炎病毒，丙型肝炎病毒，聚合酶链反应，变异 |
HEPATITISBVIRUSINFECTIONANDITSPRECORE/COREGENEMUTATIONSINCHRONICLIVERDISEASEWITHHBsAg-NEGATIVEANDANTI-HCV-POSITIVE |
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| QiuWanglong,LiFuyuan,GuonQin,et al.. HEPATITISBVIRUSINFECTIONANDITSPRECORE/COREGENEMUTATIONSINCHRONICLIVERDISEASEWITHHBsAg-NEGATIVEANDANTI-HCV-POSITIVE[J]. Chinese journal of hepatology, 1996, 4(4): 220-222 |
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| Authors: | QiuWanglong LiFuyuan GuonQin et al. |
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| Affiliation: | QiuWanglong,LiFuyuan,GuonQin,etal.DepartmentofInfectiousDisesse,GuangzhouRailwayCen-tralHospital,Guangzhou510080 |
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| Abstract: | bjectiveTostudythesignificancesofhepatitisBvirus(HBV)genemutationinchronicliverdiseasewithHBsAg-negativeandanti-HCVpositive.MethodsIn56patientswithchronicliverdis-ease,6caseswithHBsAg-negativeandanti-HCV-positive(groupA).19caseswithHBsAgandanti-HCVpotive(groupB)and31caseswithHBValone(groupC),ofwhichtheprecorecodon28stopmutant(A83)andcoremutantofcodon97leucinesubstituteforisoleucine(L97)wereanalysedbycombiningnestedpolymerasechainreaction(PCR)withrestrictionfragmentlengthpolymorphism.ResultsThedetectionratesofA83andL97ingroupAandgroupBwere33%* and42%,significantlylowerthanthoseof81%* ingroupC(P<0.001):buttheratedifferencebetweengroupAandgroupBwasnotsignificant(P>0.05).ConclusionTheresultssuggestthattheHBsAgnegativeindualinfec-tionwithHBVandHCVwasirrelativewiththemutationsofA83sndL97. |
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| Keywords: | HepatitisBvirusHepatitisCvirusPolymerasechainreactionMutation |
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