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人参茎叶霜冻前后的质量变化
引用本文:赵岩,马爽,蔡恩博,刘双利,杨鹤,张连学,王士杰. 人参茎叶霜冻前后的质量变化[J]. 中国中药杂志, 2014, 39(16): 3117-3122
作者姓名:赵岩  马爽  蔡恩博  刘双利  杨鹤  张连学  王士杰
作者单位:吉林农业大学 中药材学院, 吉林 长春 130118;吉林农业大学 中药材学院, 吉林 长春 130118;吉林农业大学 中药材学院, 吉林 长春 130118;吉林农业大学 中药材学院, 吉林 长春 130118;吉林农业大学 中药材学院, 吉林 长春 130118;吉林农业大学 中药材学院, 吉林 长春 130118;吉林农业科技学院 中药学院, 吉林 吉林 132101
基金项目:国家科技支撑计划项目(2011BAI03B01);国家公益性行业科研专项(201303111);吉林省科技发展计划项目(20126046,20140204013YY);吉林省中医药产业发展专项(YYZX201258);国家自然科学基金项目(31000154)
摘    要:
采用HPLC测定人参茎叶受冻前后单体皂苷、游离氨基酸含量,紫外分光光度法测定总酚含量及DPPH法测定自由基清除能力的变化,考察人参茎叶霜冻前后的质量变化。结果表明,受冻后人参茎中9种单体皂苷含量除Rg1,Re升高外,其余皂苷含量均有所下降,人参叶中皂苷含量均明显降低;受冻后人参茎中游离氨基总含量降低,而人参叶氨基酸总含量升高;受冻后人参茎、叶中的总酚含量降低,但对自由基的清除能力提高。霜冻对人参茎叶质量产生一定的影响。

关 键 词:人参茎叶  霜冻  人参皂苷  氨基酸  DPPH清除率
收稿时间:2014-05-19

Research on quality changes in ginseng stems and leaves before and after frost
ZHAO Yan,MA Shuang,CAI En-bo,LIU Shuang-li,YANG He,ZHANG Lian-xue and WANG Shi-jie. Research on quality changes in ginseng stems and leaves before and after frost[J]. China Journal of Chinese Materia Medica, 2014, 39(16): 3117-3122
Authors:ZHAO Yan  MA Shuang  CAI En-bo  LIU Shuang-li  YANG He  ZHANG Lian-xue  WANG Shi-jie
Affiliation:Chinese Medicine Material College of Jilin Agricultural University, Changchun 130118, China;Chinese Medicine Material College of Jilin Agricultural University, Changchun 130118, China;Chinese Medicine Material College of Jilin Agricultural University, Changchun 130118, China;Chinese Medicine Material College of Jilin Agricultural University, Changchun 130118, China;Chinese Medicine Material College of Jilin Agricultural University, Changchun 130118, China;Chinese Medicine Material College of Jilin Agricultural University, Changchun 130118, China;College of Chinese Medicine, Jilin Academy of Agricultural Science and Technology, Jilin 132101, China
Abstract:
The present study is to investigate the quality changes of ginseng stems and leaves before and after frost. The contents changes of ginsenoside,free amino acid,and total phenolic compounds, as well as DPPH radical scavenging effect before and after frost were measured. The content of 9 ginsenoside monomer in ginseng stems was decreased except for Rg1 and Re after frost, but in ginseng leaves was all decreased. The total content of amino acids was decreased in ginseng stems after frost,while increased in ginseng leaves. The content of phenolic compounds in ginseng stems and leaves were both decreased after frost while the ability of DPPH radical scavenging was improved.The factor of frost has great impact on the quality of ginseng stems and leaves.
Keywords:ginseng stems and leaves  frost  ginsenoside  amino acid  DPPH radical scavenging
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