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| 不同状态下牙髓组织蛋白质表达的二维电泳分析 | |
| 引用本文： | 林晨,聂敏,张露,陈智. 不同状态下牙髓组织蛋白质表达的二维电泳分析[J]. 中华口腔医学杂志, 2008, 43(3): 154-156 |
| 作者姓名： | 林晨 聂敏 张露 陈智 |
| 作者单位： | 武汉大学口腔医学院口腔生物医学工程教育部重点实验室,430079 |
| 基金项目： | 湖北省杰出青年科学基金 |
| 摘 要： | 目的 利用蛋白质组技术探讨牙髓对中龋和热刺激的反应.方法 用双向电泳得到牙髓在中龋和热刺激状态下的二维电泳图谱,对差异点进行质谱鉴定.结果 经Image Master2-D Platinum 5.0软件分析显示,正常牙髓和中龋牙髓的蛋白表达无显著差异;热损伤牙髓有2个蛋白点缺失,8个蛋白点下调.质谱分析鉴定了7种蛋白质.结论 本实验条件下,中龋牙髓的蛋白质表达与正常牙髓无显著差异,热刺激可造成部分蛋白表达的下调. |
| 关 键 词： | 牙髓 蛋白质组 电泳,凝胶,双向 质谱分析法 |
Two-dimensional electrophoresis analysis of the protein expression of dental pulp in different conditions |
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| LIN Chen,NIE Min,ZHANG Lu,CHEN Zhi. Two-dimensional electrophoresis analysis of the protein expression of dental pulp in different conditions[J]. Chinese journal of stomatology, 2008, 43(3): 154-156 | |
| Authors: | LIN Chen NIE Min ZHANG Lu CHEN Zhi |
| Affiliation: | Key Laboratory for Oral Biomedical Engineering of Ministry of Education, School and Hospital of Stomatology, Wuhan University, Wuhan, China. |
| Abstract: | OBJECTIVE: To analyze the different responses of dental pulp to moderate caries and thermal stimulation by proteomics. METHODS: Two-dimensional electrophoresis(2-DE) was performed to obtain the 2-D gel electrophoresis patterns of dental pulp. Mass spectrometry (MS) was used to analyze several different selected spots in the expression proteins. RESULTS: No significant difference in protein expression was found between normal and moderate carious dental. Two protein spots were absent in heat-damaged group and 8 spots showed significantly down-regulated. Seven proteins were identified by MS. CONCLUSIONS: In the present study, no significant difference in the pulp protein expression was detected between the healthy and moderate carious pulp tissues. However, down-regulation of pulp protein in thermal stimulation was observed. |
| Keywords: | Dental pulp Proteome Electrophoresis,gel,two-dimensional Mass spectrometry |
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