慢性高眼压兔模型视网膜中生长相关蛋白-43表达的变化

目的:研究持续高眼压状态下视网膜中生长相关蛋白-4(GAP-43)表达的变化。方法:兔眼前房注射复方卡波姆溶液制成慢性高眼压模型(n=21),用免疫组织化学方法观察在高眼压不同时间段视网膜中GAP-43的表达变化。结果:正常兔眼视网膜内丛状层中存在GAP-43的表达。持续性高眼压7d后内丛状层中的GAP-43免疫反应产物密度增加,且在节细胞和神经纤维层也出现阳性产物,与对照组比较有显著差异(P<0.05)。高眼压14d时阳性反应产物与7d时相比有所下降,但仍高于正常对照组(P<0.05);21d时基本恢复至正常对照组水平(P>0.05)。结论:慢性高眼压时视神经、视网膜结构受损,导致视网膜中GAP-43短暂升高。

Expression of GAP-43 in the retina of rabbit subjected to experimental ocular hypertension

·AIM:Growth-associated protein-43(GAP-43)plays an important role in the development and regeneration of central nervous system.In the present study,we examined the alteration of GAP-43 expression in the rabbit retina under experimental persisted ocular hypertension..METHODS:A chronic ocular hypertension model was established in the rabbit by an injection of Carbomer compound solution into anterior chamber.The expressions of GAP-43 in the retina were observed by means of immunohistochemistry at different time points after the ocular hypertension was produced..RESULTS:A moderate expression of GAP-43 was observed in the inner plexiform layer(IPL)of the retina in normal rabbit eyes.Seven days after ocular hypertension,the density of positive reaction products was significantly increased in IPL compared with control group(P<0.05).Some neurons in ganglion cell layer(GCL)and nerve fibers in the innermost layer(fibrous layer)of the retina were also strongly positive.Fourteen days after ocular hypertension,the positive reaction products decreased compared with those in 7-day group,but still higher than that in normal control(P<0.05).At the twenty-first day after ocular hypertension,the GAP-43 level in the retina recovered to the level of normal control..CONCLUSION:During chronic ocular hypertension,damage of retina and optic nerve triggers a transient up-regulation of GAP-43 expression..