| IL-6对人精子顶体反应影响机制的初步探讨 |
| |
| 引用本文： | 资捷,宋平.IL-6对人精子顶体反应影响机制的初步探讨[J].中华男科学杂志,2006,12(6):528-530. |
| |
| 作者姓名： | 资捷 宋平 |
| |
| 作者单位： | 1. 深圳市福田妇幼保健院,检验科,广东,深圳,518026
2. 深圳市福田妇幼保健院,生殖泌尿中心,广东,深圳,518026 |
| |
| 摘 要： | 目的:探讨IL-6对人精子顶体反应(AR)的影响机制。方法:采用BAEE/ADH法测定精子顶体酶的活性,以及通过FITC-PSA法检测精子顶体反应。结果:IL-6可诱导精子顶体酶及超氧化物歧化酶(SOD)的活性,促进精子顶体反应;胞外Ca2+单独不能诱导精子顶体反应,且没有胞外Ca2+的参与,IL-6也不能诱导精子顶体反应;蛋白激酶C(PKC)抑制剂calphC能逆转IL-6诱导的精子顶体反应。结论:IL-6对精子顶体反应有一定的促进作用,可能通过诱导精子的顶体酶和SOD活性等途径来实现,在此作用中,也涉及了PKC的激活,且还需要外源性Ca2+的参与。
|
| 关 键 词： | 白细胞介素6 精子 顶体反应 顶体酶 超氧化物歧化酶 |
| 文章编号： | 1009-3591（2006）06-0528-03 |
| 收稿时间： | 2005-10-10 |
| 修稿时间： | 2006-01-05 |
Mechanism of IL-6 on Acrosome Reaction in Human Sperm |
| |
| ZI Jie,SONG Ping.Mechanism of IL-6 on Acrosome Reaction in Human Sperm[J].National Journal of Andrology,2006,12(6):528-530. |
| |
| Authors: | ZI Jie SONG Ping |
| |
| Institution: | 1. Clinical Laboratory, 2. Centre of Urology and Reproduction, Shenzhen Futian Women and Children Health Care Hospital, Shenzhen , Guangdong 518026, China |
| |
| Abstract: | Objective: To explore the mechanism of interleukin-6(IL-6) on acrosome reaction(AR) in human sperm.Methods: Sperm acrosin activity was measured by BAEE/ADH and AR evaluated by FITC-PSA.Results: IL-6 could induce the activity of acrosin and superoxide dismutase(SOD) and enhance AR in human sperm.AR was not induced by extracellular Ca~(2 ),and IL-6-induced AR did not occur in Ca~(2 )-free medium.Calphlatin C,one inhibitor of the protein kinase C(PKC),could block IL-6-induced AR in human sperm.Conclusion: IL-6 could induce AR by stimulating the activity of acrosin and SOD in human sperm,which also involves the activation of PKC,and requires the presence of extracellular Ca~(2 ).Natl J Androl,2006,12(6):528-530 |
| |
| Keywords: | interleukin-6(IL-6) sperm acrosome reaction acrosin superoxide dismutase |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
