|
|||||
|
|
| 桂枝汤水提液中总多糖含量的测定 | |
| 引用本文： | 牛晓红,柏冬,宋剑南. 桂枝汤水提液中总多糖含量的测定[J]. 中国实验方剂学杂志, 2013, 19(13): 66-68 |
| 作者姓名： | 牛晓红 柏冬 宋剑南 |
| 作者单位： | 1. 中国中医科学院医学实验中心,北京,100700 2. 中国中医科学院中医基础理论研究所,北京,100700 |
| 基金项目： | 国家自然科学基金项目(30572334) |
| 摘 要： | 目的:建立分光光度法测定桂枝汤水提液中总多糖含量的方法.方法:以葡萄糖为对照品,苯酚-硫酸比色法测定桂枝汤水提液中总多糖的含量.结果:葡萄糖在19.62 ～ 117.70 mg·L-1与吸光度成良好线性关系,平均回收率为99.3％,RSD 2.5％ (n =6).结论:该方法简便易行,重复性良好,可作为桂枝汤水提液中总多糖含量测定的方法. |
| 关 键 词： | 桂枝汤水提液 总多糖 分光光度法 苯酚-硫酸法 含量测定 |
| 收稿时间： | 2012-05-02 |
Content Determination of Total Polysaccharide in the Guizhitang Decoction by Colorimetry |
|
| NIU Xiao-hong,BAI Dong and SONG Jian-nan. Content Determination of Total Polysaccharide in the Guizhitang Decoction by Colorimetry[J]. China Journal of Experimental Traditional Medical Formulae, 2013, 19(13): 66-68 | |
| Authors: | NIU Xiao-hong BAI Dong SONG Jian-nan |
| Affiliation: | Experiment Research Center, China Academy of Chinese Medical Sciences, Beijing 100700, China;Institute o f Basic Theory, China Academy of Chinese Medical Sciences, Beijing 100700, China;Institute o f Basic Theory, China Academy of Chinese Medical Sciences, Beijing 100700, China |
| Abstract: | Objective:To develop a method for content determination of total polysaccharide in Guizhitang decoction. Method: Glucose was used as the reference substance, and phenol-sulfuric acid was applied as coloring reagent to determine the content of total polysaccharide. Result: The calibration curve of glucose showed good linearity in the range of 19.62-117.70 mg·L-1. The average recovery rate was 99.28%, and RSD was 2.5% (n=6). Conclusion: The determination method is simple, sensitive and accurate, which can be used for evaluating the content of total polysaccharide in Guizhitang decoction. |
| Keywords: | Guizhitang decoction total polysaccharide colorimetry phenol-sulfuric acid content determination |
| 本文献已被 万方数据 等数据库收录！ | |
| 点击此处可从《中国实验方剂学杂志》浏览原始摘要信息 | |
| 点击此处可从《中国实验方剂学杂志》下载全文 | |