肺癌和乳腺癌患者外周血中CD4+CD25+T细胞及foxp3 mRNA的变化特点及临床意义

目的：探讨肺癌、乳腺癌患者外周血中CD4+CD25+调节性T细胞水平的变化及临床意义。方法：应用流式细胞术检测9例肺癌、9例乳腺癌患者及10例非肿瘤患者外周血单个核细胞(PBMC)中CD4+CD25+T细胞占CD4+T细胞的比例，应用RT-PCR技术检测人外周血PBMC foxp3基因的表达。结果：流式细胞术检测显示，肺癌及乳腺癌患者外周血PBMC中CD4+CD25+T细胞占CD4⁺T细胞的比例［（21.30±11.26）%］高于非肿瘤患者［(14.19±8.56）%］（P<0.05），肺癌及乳腺癌患者外周血PBMC中foxp3 mRNA的表达水平高于非肿瘤患者表达水平（P<0.05），肺癌和乳腺癌患者外周血CD4⁺CD25⁺调节性T细胞比例增加。结论：CD4+CD25+调节性T细胞在维持自身免疫稳定的同时对肿瘤免 疫具有抑制作用，该细胞群比例的增加可能参与肿瘤的发生。

Changes of CD4+ CD25+ T cells and expression of foxp3 mRNA in peripheral blood mononuclear cell from patients with lung cancer and breast cancer

Objective To investigate the changes of CD4~+CD25~+ regulator T cells in peripheral blood from the paitents with lung cancer and breast cancer. Methods Flow cytometry was used to analyze the proportion of CD4~+CD25~+ T cells/CD4~+T cells in peripheral blood mononuclear cells (PBMC) from 9 patients with lung cancer, 9 patients with breast cancer and 10 patients without cancer as controls. The expression of foxp3 mRNA in PBMCwas detected by RT-PCR. Results The value of CD4~+CD25~+ T cells/ CD4~+T cells in PBMC from patients with lung cancer and breast cancer (21.30±11.26)% was significantly higher compared with controls (14.19±8.56)%. The expression of foxp3 in PBMC enhanced in patients with cancer compared with controls,there was significant difference between two groups (P<0.05). It revealed that the population of CD4~+CD25~+ regulator T cells in peripheral blood of patients with cancer was increased. Conclusion CD4~+CD25~+ regulator T cells may be responsible for immune suppression in the tumor immunity while maintenance of immune tolerance and the increase of CD4~+CD25~+ regulator T cells may be involved in tumor generation.